Fluorescence detection of α-amylase based on a host–guest complex between a pyrene-derived amphiphile and γ-cyclodextrin

IF 4.6 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-10-15 Epub Date: 2025-04-17 DOI:10.1016/j.saa.2025.126219

Longjun Xiong, Yu Liu, Yutian Jiao, Gongli Wei, Baocai Xu, Fu Han, Li Zhao

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Abstract

A fluorescence detection platform for α-amylase was developed by exploiting the host–guest inclusion complex formed between γ-cyclodextrin (γ-CD) and a pyrene-functionalized amphiphile (P10CG). Spectroscopic analysis revealed that the pyrene moiety was encapsulated within the γ-CD cavity in a 2:2 stoichiometric ratio, inducing characteristic excimer fluorescence. Upon enzymatic hydrolysis of γ-CD by α-amylase, the subsequent release of P10CG led to a marked decrease in excimer emission intensity. A quantitative linear relationship (R² > 0.99) was observed between the monomer-to-excimer intensity ratio and α-amylase concentrations ranging from 1 to 5 U/mL, with a calculated detection limit of 0.135 U/mL (S/N = 3). The γ-CD/P10CG supramolecular system demonstrated satisfactory sensitivity and selectivity for α-amylase in both buffer solutions and diluted serum matrices, thus providing a potential sensing system for α-amylase in complex biological systems.

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基于芘衍生的两亲体和γ-环糊精之间的主-客体配合物的α-淀粉酶荧光检测

利用γ-环糊精（γ-CD）与芘功能化两亲体（P10CG）之间形成的主客体包合物，建立了α-淀粉酶的荧光检测平台。光谱分析表明，芘部分以2:2的化学计量比被包裹在γ-CD腔内，引起特征准分子荧光。α-淀粉酶水解γ-CD后，随后释放的P10CG导致准分子发射强度显著降低。定量线性关系(R2 >；α-淀粉酶浓度范围为1 ~ 5 U/mL，计算检出限为0.135 U/mL （S/N = 3）。γ-CD/P10CG超分子体系对α-淀粉酶在缓冲液和稀释血清基质中均表现出满意的灵敏度和选择性，为复杂生物体系中α-淀粉酶的检测提供了一种潜在的检测体系。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.