Recent advances of capillary electrophoresis for enzyme analysis (2021–2024)

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchemical Journal Pub Date : 2025-06-01 Epub Date: 2025-04-18 DOI:10.1016/j.microc.2025.113701

Yangyang Su , Fan Shui , Jia Tang, Yanping Wei, Qian Zhang, Famin Ke, Jing Zeng

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Abstract

Capillary electrophoresis (CE) is a robust and versatile analytical technique that has demonstrated significant potential and emerging trends in enzyme analysis. This review comprehensively summarizes the latest advancements in CE for enzyme analysis from 2021 to 2024, covering both homogeneous and heterogeneous analysis. The advancements in homogeneous CE enzyme analysis, including both pre-capillary enzyme analysis and in-capillary enzyme analysis, are thoroughly examined. Specifically, an in-depth analysis is provided of the general principles, characteristics, and key parameters at each step of the pre-capillary analysis process, such as incubation, termination, separation, and detection. Moreover, this review introduces various novel developments and applications in different mixed modes of in-capillary enzyme analysis. Additionally, it discusses diverse immobilization methods, such as adsorption, cross-linking, covalent bonding, encapsulation, and the recently developed nanogels method, aiming to construct highly efficient and stable immobilized enzyme microreactors (IMERs). This review presents an up-to-date overview of CE applications in enzyme analysis, highlighting its significant potential in this field.

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毛细管电泳酶分析研究进展（2021-2024）

毛细管电泳（CE）是一种强大而通用的分析技术，在酶分析中显示出巨大的潜力和新兴趋势。本文全面总结了从2021年到2024年CE在酶分析方面的最新进展，包括均相分析和异质分析。均相CE酶分析的进展，包括毛细管前酶分析和毛细管内酶分析，进行了全面的审查。具体来说，深入分析了毛细管前分析过程的一般原理、特征和每个步骤的关键参数，如孵育、终止、分离和检测。此外，本文还介绍了毛细管内酶分析在不同混合模式下的最新进展和应用。此外，本文还讨论了吸附法、交联法、共价键法、包封法以及最近发展起来的纳米凝胶法等多种固定化方法，旨在构建高效、稳定的固定化酶微反应器。本文综述了CE在酶分析中的最新应用，强调了其在该领域的重大潜力。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.