Correction to “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels”

IF 3.6 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2025-04-18 DOI:10.1002/bit.29007
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引用次数: 0

Abstract

Y. Du, M. Ghodousi, H. Qi, N. Haas, W. Xiao, and A. Khademhosseini, “Sequential Assembly of Cell-Laden Hydrogel Constructs to Engineer Vascular-Like Microchannels,” Biotechnology and Bioengineering 108, no. 7 (2011): 1693–1703, 10.1002/bit.23102.

This correction is published due to concerns raised by a third party regarding high similarity between the cell-laden microgels between Figure 4B,D.

The authors explained that the images in Figure 4B,D were from the same sample that was sequentially exposed to different conditions (first exposed to PI and subsequently put in oil) and analyzed. Thus, the microgels appeared repositioned between the imaging sessions. Due to the elapsed time since publication, the original image files were not available, therefore the authors have repeated the experiment in which the gels were not sequentially analyzed to verify the conclusion that “each assembly phase is conducive to cell viability.”

The new data confirmed the same trends as observed before, therefore the experimental results and the corresponding conclusions of the paper remain unaffected.

The corrected Figure 4 is below:

Abstract Image

Abstract Image

Abstract Image

Abstract Image

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更正“装载细胞的水凝胶结构的顺序组装以工程血管样微通道”
杜毅,齐海峰,肖伟,“细胞负载水凝胶结构的序列组装及其在血管样微通道中的应用”,《生物工程学报》,2008,no。[7] (2011): 1693-1703, 10.1002/bit.23102。由于第三方对图4B、D中载细胞微凝胶之间的高度相似性提出了担忧,因此发表了这一更正。作者解释说,图4B,D中的图像来自于同一样品,该样品依次暴露于不同的条件下(首先暴露于PI,然后放入油中)并进行分析。因此,微凝胶在成像期间出现了重新定位。由于发表后已经过了一段时间,原始图像文件不可用,因此作者重复了实验,其中凝胶没有顺序分析,以验证“每个组装阶段都有利于细胞活力”的结论。新数据证实了之前观察到的相同趋势,因此实验结果和论文的相应结论不受影响。修正后的图4如下:图4在图查看器中打开powerpoint负载细胞的管状水凝胶的活力测试,作为组装过程中步骤的函数。顺序组装过程被证明是细胞友好的,正如活力测试所证明的:(A) 3T3装载成纤维细胞的微凝胶;(B)微凝胶用光引发剂(PI) (1% DPBS)洗涤三次;(C)暴露于油中的微凝胶;(D)同时暴露于PI和油中的微凝胶;(E)培养基中2天后的微凝胶组装;(F)每个制备步骤后对样品(n = 3)的定量分析表明,装配过程不会导致细胞活力的显著丧失。比例尺:500µm。
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来源期刊
Biotechnology and Bioengineering
Biotechnology and Bioengineering 工程技术-生物工程与应用微生物
CiteScore
7.90
自引率
5.30%
发文量
280
审稿时长
2.1 months
期刊介绍: Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.
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