Deuterium stress Reprograms Chlorella sorokiniana Metabolism: Coupling photosynthetic suppression with carbon Reserve Surge

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2025-08-01 Epub Date: 2025-04-14 DOI:10.1016/j.biortech.2025.132548

Tianfei Li, Yixian He, Shanni Ke, Zexia Cai, Zhuo Jiang

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Abstract

Microalgae convert inorganic substrates into stable isotope-labeled compounds, ideal for deuterated compound production. However, the mechanism by which deuterium affects the growth and metabolism of microalgae remains unclear. This study aims to reveal the effects of deuterium on the growth and metabolic processes of Chlorella sorokiniana and to clarify the interaction between them. After deuterium treatment, cell growth and the accumulation of photosynthetic pigments were significantly inhibited, leading to reduced photosynthetic efficiency and blocked energy transfer. Under 100% D₂O conditions, the accumulation of starch and lipids was enhanced, with starch content reaching up to 52% of dry cell weight and lipid content reaching 22%. Transcriptomics revealed deuterium stress inhibited photosynthesis-related genes while upregulating pathways for starch, fatty acid, and nucleic acid synthesis. These findings reveal the adaptation mechanism of microalgae to deuterium treatment and provide valuable insights for the utilization of microalgae in the production of deuterated organic compounds.

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氘胁迫重编程小球藻代谢：光合抑制与碳储备激增的耦合

微藻将无机底物转化为稳定的同位素标记化合物，是生产氘化化合物的理想选择。然而，氘对微藻生长和代谢的影响机制尚不清楚。本研究旨在揭示氘对sorokiniana小球藻生长和代谢过程的影响，并阐明它们之间的相互作用。氘处理后，细胞生长和光合色素积累明显受到抑制，导致光合效率降低，能量传递受阻。在100% D2O条件下，淀粉和脂肪的积累增强，淀粉含量可达干细胞重的52%，脂肪含量可达22%。转录组学显示，氘胁迫抑制了光合作用相关基因，上调了淀粉、脂肪酸和核酸合成途径。这些发现揭示了微藻对氘处理的适应机制，为利用微藻生产氘化有机化合物提供了有价值的见解。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.