First characterization of four repeat regions with the blaNDM-1 carried on an IncFII plasmid in Enterobacter hormaechei

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2025-04-08 DOI:10.1016/j.isci.2025.112369

Yi Liu , Xiaojing Liu , Ruishan Liu , Hao Xu , Mantao Chen , Jiajie Qian , Beiwen Zheng

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Abstract

Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four bla_NDM-1 genes in tandem on an IncFII plasmid from Enterobacter hormaechei. Whole-genome sequencing (WGS) of E. hormaechei isolates L710hy identified a four-tandem repeat of the bla_NDM-1 gene, with each copy’s environment nearly identical (ISCR1-aph(3′)-VI-ISAba125-bla_NDM-1-ble-iso-tat-dsbD-cutA-sul1-ISCR1). The study suggests a correlation between the bla_NDM-1 copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four bla_NDM-1 tandem repeat structures was also investigated. This is the first documented case of bla_NDM-1 tandem repeats in E. hormaechei, and the ISCR1 and other insertion sequence may play a role in this arrangement.

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首次鉴定了宿主肠杆菌IncFII质粒携带blaNDM-1的4个重复区

碳青霉烯耐药肠杆菌科（CRE）已成为对全球健康的严重威胁，多拷贝耐药基因是增加耐药性的潜在因素。本研究研究了四种blaNDM-1基因在激素肠杆菌IncFII质粒上串联的新排列。全基因组测序（WGS）鉴定出了一个blaNDM-1基因的四串联重复序列，每个拷贝的环境几乎相同（ISCR1-aph(3 ')- vi - isaba125 -blaNDM-1-ble-iso- stat - dsbd - cuta -sul1- iscr1）。该研究提示blaNDM-1拷贝数与碳青霉烯类的最低抑制浓度（MIC）值之间存在相关性，但需要进一步的研究来证实这种相关性。并对这4个blaNDM-1串联重复结构的稳定性进行了研究。这是首次在荷氏肠杆菌中发现blaNDM-1串联重复序列，ISCR1和其他插入序列可能在这种排列中起作用。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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