Exploring the bioactive potential of chitosan extracted from Portunus trituberculatus crab shell for multifaceted applications: Antioxidant, antidiabetic, anti-inflammatory and cytotoxic activities

IF 2.7 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Materials Letters Pub Date : 2025-04-19 DOI:10.1016/j.matlet.2025.138581

M.Meenakshi Sundari , A. Jegatheesan , R. Mohan , M.Sam Arul Raj , N.Ambika Devi , P. Ravikumar , M. Ayyanar , K. Ravichandran

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Abstract

Chitosan (CS) is a natural polysaccharide with significant potential for biological applications. This study aims to extract chitosan from crab shell (Portunus trituberculatus) waste through a series of processes including demineralization, deproteinization, and deacetylation. The crystallinity index and deacetylation degree (DD) were determined, and the chitosan was characterized using several analytical techniques, viz. XRD, FTIR, Raman spectroscopy and SEM with EDS. The crab shell derived chitosan exhibits significant antioxidant, antidiabetic and anti-inflammatory properties. The CS showed 90.35 % inhibition in ABTS free radical scavenging assays and showed 88.40 % of inhibition against the alpha glucosidase enzyme. It also showed an anti-inflammation potential with the IC₅₀ value of 132.72 ± 1.36 µg/mL. Furthermore, the cytotoxicity test of the CS against MCF – 7 cell line exhibited an IC₅₀ value of 22.86 ± 0.05 µg/mL.

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探讨三管蟹壳壳聚糖在抗氧化、抗糖尿病、抗炎和细胞毒性等方面的生物活性潜力

壳聚糖（CS）是一种具有重要生物应用潜力的天然多糖。本研究旨在从蟹壳（Portunus trituberculatus）废料中通过脱矿、脱蛋白、脱乙酰等一系列工艺提取壳聚糖。测定了壳聚糖的结晶度和去乙酰化度，并用XRD、FTIR、拉曼光谱、扫描电镜和能谱仪对壳聚糖进行了表征。蟹壳壳聚糖具有显著的抗氧化、抗糖尿病和抗炎作用。CS对ABTS自由基清除的抑制率为90.35%，对α -葡萄糖苷酶的抑制率为88.40%。其IC50值为132.72±1.36µg/mL。此外，CS对MCF - 7细胞株的细胞毒性试验显示，IC50值为22.86±0.05µg/mL。

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来源期刊

Materials Letters 工程技术-材料科学：综合

CiteScore

5.60

自引率

3.30%

发文量

1948

审稿时长

50 days

期刊介绍： Materials Letters has an open access mirror journal Materials Letters: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Materials Letters is dedicated to publishing novel, cutting edge reports of broad interest to the materials community. The journal provides a forum for materials scientists and engineers, physicists, and chemists to rapidly communicate on the most important topics in the field of materials. Contributions include, but are not limited to, a variety of topics such as: • Materials - Metals and alloys, amorphous solids, ceramics, composites, polymers, semiconductors • Applications - Structural, opto-electronic, magnetic, medical, MEMS, sensors, smart • Characterization - Analytical, microscopy, scanning probes, nanoscopic, optical, electrical, magnetic, acoustic, spectroscopic, diffraction • Novel Materials - Micro and nanostructures (nanowires, nanotubes, nanoparticles), nanocomposites, thin films, superlattices, quantum dots. • Processing - Crystal growth, thin film processing, sol-gel processing, mechanical processing, assembly, nanocrystalline processing. • Properties - Mechanical, magnetic, optical, electrical, ferroelectric, thermal, interfacial, transport, thermodynamic • Synthesis - Quenching, solid state, solidification, solution synthesis, vapor deposition, high pressure, explosive