Characterization of a novel 1,2-dichloroethane degrader Ancylobacter sp. J3 and use of its immobilized cells in the treatment of polluted groundwater

Abstract

A novel 1,2-dichloroethane (1,2-DCA) degrading bacteria strain J3 was isolated from 1,2-DCA contaminated groundwater and identified as Ancylobacter sp. The strain J3 was associated with self-flocculation during the growth process, and the degradation pathway study showed that the bacteria could completely mineralize 1,2-DCA. The microorganism was immobilized and the optimum preparation conditions were obtained by orthogonal experiment: 6 % polyvinyl alcohol, 2 % sodium alginate, 1 % biochar, and 2 % CaCl_2, and the immobilized cells were named J3C. The degradation rates of J3C at low pH, temperature, and high concentration NaCl were higher than that of free J3. The fitting results of the pseudo-first-order degradation kinetics model showed that for above 200 mg/L 1,2-DCA, the degradation rate of J3C was higher than that of free J3. The adsorption process of the sterile J3C aligns with the pseudo-first-order kinetic model and the intraparticle diffusion model. The internal mass transfer kinetics analysis revealed that the beads with biochar and a small diameter (0.34 cm) were more conducive to mass transfer. Finally, remediation of real polluted groundwater by J3C shows that for groundwater with a pH value of about 7, 1,2-DCA concentrations of about 100, 200 mg/L, 1,2-DCA can be completely degraded by J3C, while for groundwater with a pH value of 12, 250 mg/L 1,2-DCA, the degradation rate was 83.15 % by J3C, 66.91 % higher than that of free J3. The changes in microbial communities in groundwater showed that J3C disturbed the groundwater microbial little for the immobilized cells in J3C originated from the groundwater.