{"title":"Overexpression of FvGCN5 enhances the resistance of woodland strawberry against Botrytis cinerea","authors":"Miao Yu, Jiaqi Zhang, Feifei Bai, Yihan Gao, Shan Jiang, Hui Liu, Aisheng Xiong, Zongming Cheng, Jinsong Xiong","doi":"10.1016/j.jplph.2025.154496","DOIUrl":null,"url":null,"abstract":"<div><div>Epigenetic modifications mediated by histone acetylation play essential roles in plant development and stress response. However, the mechanism of regulating biotic stress through histone acetyltransferase GCN5 in strawberry is still unclear. In this study, we isolated <em>FvGCN5</em> from woodland strawberry and found that FvGCN5 may form the conserved SAGA (Spt-Ada-Gcn5 acetyltransferase) complex through interaction with FvADA2a and FvADA2b. In addition, we found that <em>FvGCN5</em> could be significantly induced by the infection of fungal pathogen <em>Botrytis cinerea</em>, and that the transgenic strawberry plants overexpressing <em>FvGCN5</em> exhibited enhanced resistance against <em>B. cinerea</em>. Further RNA-seq-based transcriptome and quantitative PCR analysis indicated that several disease-resistant genes such as <em>FvMYC2</em> and, <em>FvPR1</em> were significantly upregulated in <em>FvGCN5</em> overexpression lines. Taken together, our study indicates that <em>FvGCN5</em> plays important roles in the resistance against <em>B. cinerea</em> in woodland strawberry through activating disease-resistant genes.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"308 ","pages":"Article 154496"},"PeriodicalIF":4.1000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161725000781","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/16 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Epigenetic modifications mediated by histone acetylation play essential roles in plant development and stress response. However, the mechanism of regulating biotic stress through histone acetyltransferase GCN5 in strawberry is still unclear. In this study, we isolated FvGCN5 from woodland strawberry and found that FvGCN5 may form the conserved SAGA (Spt-Ada-Gcn5 acetyltransferase) complex through interaction with FvADA2a and FvADA2b. In addition, we found that FvGCN5 could be significantly induced by the infection of fungal pathogen Botrytis cinerea, and that the transgenic strawberry plants overexpressing FvGCN5 exhibited enhanced resistance against B. cinerea. Further RNA-seq-based transcriptome and quantitative PCR analysis indicated that several disease-resistant genes such as FvMYC2 and, FvPR1 were significantly upregulated in FvGCN5 overexpression lines. Taken together, our study indicates that FvGCN5 plays important roles in the resistance against B. cinerea in woodland strawberry through activating disease-resistant genes.
期刊介绍：
The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication.
The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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