A rapid, specific, and simple-to-use biosensor for amplification-free determination of microRNA based on electrical potential-assisted and ternary hybridization

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-04-22 DOI:10.1007/s00604-025-07143-8

Yaru Liu, Yang Li, Hao Gong, Yao Liu, Yijie Wang, Cuiping Ma, Yuxi Wei, Chao Shi

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Abstract

An ultra-fast, easy-to-use and non-amplification electrochemical detection platform was constructed for microRNA (miRNA) detection. A set of label-free hairpin probes and capture probes were introduced to form a ternary complex, which could enhance the selectivity and stability of miRNA detection due to the ability of reducing non-specific bind with non-target and enhancing accessibility of target to probes. Moreover, the capture probe immobilization and hybridization process were accelerated by the external electric field, shortening the detection time from 2 h to 5 min. The platform showed a detection limit of 1.28 fM under ideal experimental control conditions and had ability to identify 1- or 2-nucleotide (nt) difference. In addition, the designed sensor achieved the sensitive determination of miRNA-21 in serum samples. The excellent anti-interference capability of this detection method indicated its potential for clinical application. Its simplicity and high specificity made this method a promising tool for detecting different miRNA to assist the diagnosis of diverse cancers.

Graphical Abstract

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一种基于电势辅助和三元杂交的快速，特异性和简单易用的生物传感器，用于无扩增的microRNA测定

构建了一种超快速、易操作、无扩增的微rna （miRNA）电化学检测平台。引入一组无标记的发夹探针和捕获探针组成三元配合物，可以减少与非靶标的非特异性结合，提高靶标对探针的可及性，从而提高miRNA检测的选择性和稳定性。此外，外电场加速了捕获探针的固定化和杂交过程，将检测时间从2 h缩短到5 min。在理想的实验控制条件下，该平台的检测限为1.28 fM，能够识别1-或2-核苷酸（nt）差异。此外，所设计的传感器实现了血清样品中miRNA-21的灵敏测定。该检测方法具有良好的抗干扰能力，具有较好的临床应用前景。它的简单性和高特异性使该方法成为检测不同miRNA以辅助多种癌症诊断的有前途的工具。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.