Polylactic acid microplastics and earthworms drive cadmium bioaccumulation and toxicity in the soil–radish health community

Abstract

Recent studies underscored the toxicity of microplastics (MPs) as vectors for cadmium (Cd) in soil-plant systems, yet the driven potential of soil fauna in real-world environments remains overlooked. This study examined the interactive effects of earthworms and polylactic acid (PLA) MPs (0.5 % w/w) on rhizosphere biochemistry and Cd (2 mg/kg)-induced phytotoxicity in radish. The combined treatment of earthworms and PLA MPs significantly increased the soil available Cd (diethylenetriaminepentaacetic acid -extractable Cd) from 0.79 mg/kg to 1.01 mg/kg compared to the Cd treatment (p < 0.05) and enhanced the bacterial network stability. Cd accumulation in radish was significantly elevated under the combined treatment (roots: 2.04 mg/kg; leaves: 12.31 mg/kg) compared to the Cd treatment (roots: 1.59 mg/kg; leaves: 8.82 mg/kg) (p < 0.05). The combined treatment activated the radish antioxidant system. The combined treatment (roots: 6.08 g; leaves: 1.65 g) significantly reduced radish biomass compared to the Cd treatment (roots: 24.41 g; leaves: 4.45 g) (p < 0.05). Metabolic pathways involving lipid and carbohydrate metabolism, membrane transport, and secondary metabolite biosynthesis were disrupted. Structural equation modeling identified rhizosphere soil properties (pH, SOM, and CEC) as well as Cd and antioxidant systems in the leaf as major contributors to radish growth inhibition.