Nitrogen removal performance and feeding strategy optimisation in a nitritation denitrification step-feed sequencing batch reactor system

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biochemical Engineering Journal Pub Date : 2025-08-01 Epub Date: 2025-04-18 DOI:10.1016/j.bej.2025.109761

Yihan Wang , Zhenjun Wu , Qingwen Qin , Lijie Chen , Ming Cai , Xin Chen , Yutong Liu , Ziang Su , Xinyi Fan , Lang Cheng

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Abstract

A 90-day experiment was conducted to optimise the stability of Nitritation Denitrification and improve COD utilisation. The results showed that Nitritation Denitrification in a step-feed sequencing batch reactor using the influent-anoxic/aerobic (IA/O) mode can achieve efficient and stable nitrogen removal at C/N = 4. The removal efficiencies of NH₄⁺-N and total inorganic nitrogen (TIN) were 95 % and 85.5 % respectively. Batch experiments were conducted that an unlimited increase in the number of step feeds in a limited time would affect the nitrogen removal. Extending the duration of the aerobic phase inhibited Flavobacterium and favoured the relative abundance of the nirS and nirK genes. This study may provide a reference for optimising the Nitritation Denitrification process.

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硝化反硝化步进序批式反应器系统脱氮性能及进料策略优化

为优化硝化反硝化的稳定性，提高COD的利用率，进行了为期90 d的试验。结果表明，采用进水-缺氧/好氧（IA/O）模式的步进式序批式反应器的硝化反硝化可达到C/N = 4的高效稳定脱氮效果。对NH4+-N和总无机氮（TIN）的去除率分别为95 %和85.5 %。间歇式实验表明，在有限时间内无限增加进料次数会影响脱氮效果。延长好氧期对黄杆菌有抑制作用，有利于nirS和nirK基因的相对丰度。本研究可为优化硝化反硝化工艺提供参考。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.