Detection of multiple biometabolite molecules by SPR-SERS dual-functional biosensors based on highly sensitive ridge Hyperbolic Metamaterials

Abstract

Disease can be predicted by monitoring the concentration of metabolite molecules in human body fluids, but the current detection methods have problems such as low detection sensitivity, and they can only provide a simple piece of information, which is prone to false positives and lead to incorrect judgments. Therefore, it is essential to design a dual-functional biosensor with high sensitivity that can provide comprehensive data. In order to solve the above problems, we designed and prepared the Au-ridge Hyperbolic Metamaterials (HMMs) SPR-SERS dual-functional biosensor. Experiments and theoretical simulations have demonstrated that the dual-functional biosensor possesses both high sensitivity SPR and SERS detection capabilities, with a bulk sensitivity of 6667 nm/RIU and the SERS detection limit of 10⁻¹³ M. Furthermore, the dual-functional biosensor is highly responsive to a wide range of metabolite molecules. Finally, we used this dual-functional biosensor to obtain high-contrast SPR and SERS signals for low concentrations of urea and uric acid solutions. We believe that this dual-functional biosensor can provide a more reliable platform for the monitoring of metabolite molecules.