{"title":"Virus-induced genome editing using a miniature CRISPR system","authors":"","doi":"10.1038/s41477-025-01990-2","DOIUrl":null,"url":null,"abstract":"The tobacco rattle virus was engineered to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA. This miniature CRISPR system enabled transgene-free germline genome editing in Arabidopsis thaliana without the need for tissue culture or plant transformation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 5","pages":"951-952"},"PeriodicalIF":13.6000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Plants","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41477-025-01990-2","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The tobacco rattle virus was engineered to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA. This miniature CRISPR system enabled transgene-free germline genome editing in Arabidopsis thaliana without the need for tissue culture or plant transformation.
期刊介绍：
Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.
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