Pub Date : 2024-11-18DOI: 10.1038/s41477-024-01855-0
Shalik Ram Sigdel, Xiangyu Zheng, Flurin Babst, J. Julio Camarero, Shan Gao, Xiaoxia Li, Xiaoming Lu, Jayram Pandey, Binod Dawadi, Jian Sun, Haifeng Zhu, Tao Wang, Eryuan Liang, Josep Peñuelas
Understanding how climate change influences succession is fundamental for predicting future forest composition. Warming is expected to accelerate species succession at their cold thermal ranges, such as alpine treelines. Here we examined how interactions and successional strategies of the early-successional birch (Betula utilis) and the late-successional fir (Abies spectabilis) affected treeline dynamics by combining plot data with an individual-based treeline model at treelines in the central Himalayas. Fir showed increasing recruitment and a higher upslope shift rate (0.11 ± 0.02 m yr−1) compared with birch (0.06 ± 0.03 m yr−1) over the past 200 years. Spatial analyses indicate strong interspecies competition when trees were young. Model outputs from various climatic scenarios indicate that fir will probably accelerate its upslope movement with warming, while birch recruitment will decline drastically, forming stable or even retreating treelines. Our findings point to accelerating successional dynamics with late-successional species rapidly outcompeting pioneer species, offering insight into future forest succession and its influences on ecosystem services.
{"title":"Accelerated succession in Himalayan alpine treelines under climatic warming","authors":"Shalik Ram Sigdel, Xiangyu Zheng, Flurin Babst, J. Julio Camarero, Shan Gao, Xiaoxia Li, Xiaoming Lu, Jayram Pandey, Binod Dawadi, Jian Sun, Haifeng Zhu, Tao Wang, Eryuan Liang, Josep Peñuelas","doi":"10.1038/s41477-024-01855-0","DOIUrl":"https://doi.org/10.1038/s41477-024-01855-0","url":null,"abstract":"<p>Understanding how climate change influences succession is fundamental for predicting future forest composition. Warming is expected to accelerate species succession at their cold thermal ranges, such as alpine treelines. Here we examined how interactions and successional strategies of the early-successional birch (<i>Betula utilis</i>) and the late-successional fir (<i>Abies spectabilis</i>) affected treeline dynamics by combining plot data with an individual-based treeline model at treelines in the central Himalayas. Fir showed increasing recruitment and a higher upslope shift rate (0.11 ± 0.02 m yr<sup>−1</sup>) compared with birch (0.06 ± 0.03 m yr<sup>−1</sup>) over the past 200 years. Spatial analyses indicate strong interspecies competition when trees were young. Model outputs from various climatic scenarios indicate that fir will probably accelerate its upslope movement with warming, while birch recruitment will decline drastically, forming stable or even retreating treelines. Our findings point to accelerating successional dynamics with late-successional species rapidly outcompeting pioneer species, offering insight into future forest succession and its influences on ecosystem services.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"17 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15DOI: 10.1038/s41477-024-01857-y
Jean-David Rochaix
The pyrenoid contains internal membrane structures that are required for efficient carbon fixation. The two proteins SAGA1 and MITH1 are necessary for the biogenesis of these membranes and the delivery of bicarbonate to the pyrenoid matrix.
{"title":"New light on pyrenoid membrane tubules","authors":"Jean-David Rochaix","doi":"10.1038/s41477-024-01857-y","DOIUrl":"https://doi.org/10.1038/s41477-024-01857-y","url":null,"abstract":"The pyrenoid contains internal membrane structures that are required for efficient carbon fixation. The two proteins SAGA1 and MITH1 are necessary for the biogenesis of these membranes and the delivery of bicarbonate to the pyrenoid matrix.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"246 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-15DOI: 10.1038/s41477-024-01847-0
Jessica H. Hennacy, Nicky Atkinson, Angelo Kayser-Browne, Sabrina L. Ergun, Eric Franklin, Lianyong Wang, Simona Eicke, Yana Kazachkova, Moshe Kafri, Friedrich Fauser, Josep Vilarrasa-Blasi, Robert E. Jinkerson, Samuel C. Zeeman, Alistair J. McCormick, Martin C. Jonikas
Approximately one-third of global CO2 assimilation is performed by the pyrenoid, a liquid-like organelle found in most algae and some plants. Specialized pyrenoid-traversing membranes are hypothesized to drive CO2 assimilation in the pyrenoid by delivering concentrated CO2, but how these membranes are made to traverse the pyrenoid matrix remains unknown. Here we show that proteins SAGA1 and MITH1 cause membranes to traverse the pyrenoid matrix in the model alga Chlamydomonas reinhardtii. Mutants deficient in SAGA1 or MITH1 lack matrix-traversing membranes and exhibit growth defects under CO2-limiting conditions. Expression of SAGA1 and MITH1 together in a heterologous system, the model plant Arabidopsis thaliana, produces matrix-traversing membranes. Both proteins localize to matrix-traversing membranes. SAGA1 binds to the major matrix component, Rubisco, and is necessary to initiate matrix-traversing membranes. MITH1 binds to SAGA1 and is necessary for extension of membranes through the matrix. Our data suggest that SAGA1 and MITH1 cause membranes to traverse the matrix by creating an adhesive interaction between the membrane and matrix. Our study identifies and characterizes key factors in the biogenesis of pyrenoid matrix-traversing membranes, demonstrates the importance of these membranes to pyrenoid function and marks a key milestone toward pyrenoid engineering into crops for improving yields.
{"title":"SAGA1 and MITH1 produce matrix-traversing membranes in the CO2-fixing pyrenoid","authors":"Jessica H. Hennacy, Nicky Atkinson, Angelo Kayser-Browne, Sabrina L. Ergun, Eric Franklin, Lianyong Wang, Simona Eicke, Yana Kazachkova, Moshe Kafri, Friedrich Fauser, Josep Vilarrasa-Blasi, Robert E. Jinkerson, Samuel C. Zeeman, Alistair J. McCormick, Martin C. Jonikas","doi":"10.1038/s41477-024-01847-0","DOIUrl":"https://doi.org/10.1038/s41477-024-01847-0","url":null,"abstract":"<p>Approximately one-third of global CO<sub>2</sub> assimilation is performed by the pyrenoid, a liquid-like organelle found in most algae and some plants. Specialized pyrenoid-traversing membranes are hypothesized to drive CO<sub>2</sub> assimilation in the pyrenoid by delivering concentrated CO<sub>2</sub>, but how these membranes are made to traverse the pyrenoid matrix remains unknown. Here we show that proteins SAGA1 and MITH1 cause membranes to traverse the pyrenoid matrix in the model alga <i>Chlamydomonas reinhardtii</i>. Mutants deficient in <i>SAGA1</i> or <i>MITH1</i> lack matrix-traversing membranes and exhibit growth defects under CO<sub>2</sub>-limiting conditions. Expression of SAGA1 and MITH1 together in a heterologous system, the model plant <i>Arabidopsis thaliana</i>, produces matrix-traversing membranes. Both proteins localize to matrix-traversing membranes. SAGA1 binds to the major matrix component, Rubisco, and is necessary to initiate matrix-traversing membranes. MITH1 binds to SAGA1 and is necessary for extension of membranes through the matrix. Our data suggest that SAGA1 and MITH1 cause membranes to traverse the matrix by creating an adhesive interaction between the membrane and matrix. Our study identifies and characterizes key factors in the biogenesis of pyrenoid matrix-traversing membranes, demonstrates the importance of these membranes to pyrenoid function and marks a key milestone toward pyrenoid engineering into crops for improving yields.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"25 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-09DOI: 10.1038/s41477-023-01564-0
Mathilde Arrivé, Mathieu Bruggeman, Vasileios Skaltsogiannis, Léna Coudray, Yi-Fat Quan, Cédric Schelcher, Valérie Cognat, Philippe Hammann, Johana Chicher, Philippe Wolff, Anthony Gobert, Philippe Giegé
RNase P is the essential activity that performs the 5′ maturation of transfer RNA (tRNA) precursors. Beyond the ancestral form of RNase P containing a ribozyme, protein-only RNase P enzymes termed PRORP were identified in eukaryotes. In human mitochondria, PRORP forms a complex with two protein partners to become functional. In plants, although PRORP enzymes are active alone, we investigate their interaction network to identify potential tRNA maturation complexes. Here we investigate functional interactions involving the Arabidopsis nuclear RNase P PRORP2. We show, using an immuno-affinity strategy, that PRORP2 occurs in a complex with the tRNA methyl transferases TRM1A and TRM1B in vivo. Beyond RNase P, these enzymes can also interact with RNase Z. We show that TRM1A/TRM1B localize in the nucleus and find that their double knockout mutation results in a severe macroscopic phenotype. Using a combination of immuno-detections, mass spectrometry and a transcriptome-wide tRNA sequencing approach, we observe that TRM1A/TRM1B are responsible for the m22G26 modification of 70% of cytosolic tRNAs in vivo. We use the transcriptome wide tRNAseq approach as well as RNA blot hybridizations to show that RNase P activity is impaired in TRM1A/TRM1B mutants for specific tRNAs, in particular, tRNAs containing a m22G modification at position 26 that are strongly downregulated in TRM1A/TRM1B mutants. Altogether, results indicate that the m22G-adding enzymes TRM1A/TRM1B functionally cooperate with nuclear RNase P in vivo for the early steps of cytosolic tRNA biogenesis. This study shows that the tRNA-modifying enzymes TRM1A/TRM1B are essential to attain the steady-state pool of tRNAs and reveals how they functionally cooperate with RNase P in vivo for the early steps of tRNA biogenesis in Arabidopsis.
RNase P是进行转移RNA（tRNA）前体5′成熟的必需活性。除了含有核酶的RNase P的祖先形式外，在真核生物中还鉴定出了称为PRORP的纯蛋白质RNase P酶。在人类线粒体中，PRORP与两个蛋白质伴侣形成复合物，从而发挥功能。在植物中，尽管PRORP酶单独具有活性，但我们研究了它们的相互作用网络，以确定潜在的tRNA成熟复合物。在这里，我们研究了涉及拟南芥核RNase P PRORP2的功能相互作用。我们使用免疫亲和策略表明，PRORP2在体内与tRNA甲基转移酶TRM1A和TRM1B形成复合物。除了RNase P，这些酶还可以与RNase Z相互作用。我们发现TRM1A/TRM1B定位在细胞核中，并发现它们的双敲除突变导致严重的宏观表型。通过结合免疫检测、质谱和转录组范围的tRNA测序方法，我们观察到TRM1A/TRM1B负责体内70%胞质tRNA的m22G26修饰。我们使用转录组范围的tRNAseq方法以及RNA印迹杂交来表明，对于特定的tRNA，特别是在TRM1A/TRM1B突变体中强烈下调的26位含有m22G修饰的tRNAs，在TRM1A/4RM1B突变体中RNase P活性受损。总之，结果表明，m22G添加酶TRM1A/TRM1B在体内与核RNase P功能性地协同进行胞质tRNA生物发生的早期步骤。
{"title":"A tRNA-modifying enzyme facilitates RNase P activity in Arabidopsis nuclei","authors":"Mathilde Arrivé, Mathieu Bruggeman, Vasileios Skaltsogiannis, Léna Coudray, Yi-Fat Quan, Cédric Schelcher, Valérie Cognat, Philippe Hammann, Johana Chicher, Philippe Wolff, Anthony Gobert, Philippe Giegé","doi":"10.1038/s41477-023-01564-0","DOIUrl":"10.1038/s41477-023-01564-0","url":null,"abstract":"RNase P is the essential activity that performs the 5′ maturation of transfer RNA (tRNA) precursors. Beyond the ancestral form of RNase P containing a ribozyme, protein-only RNase P enzymes termed PRORP were identified in eukaryotes. In human mitochondria, PRORP forms a complex with two protein partners to become functional. In plants, although PRORP enzymes are active alone, we investigate their interaction network to identify potential tRNA maturation complexes. Here we investigate functional interactions involving the Arabidopsis nuclear RNase P PRORP2. We show, using an immuno-affinity strategy, that PRORP2 occurs in a complex with the tRNA methyl transferases TRM1A and TRM1B in vivo. Beyond RNase P, these enzymes can also interact with RNase Z. We show that TRM1A/TRM1B localize in the nucleus and find that their double knockout mutation results in a severe macroscopic phenotype. Using a combination of immuno-detections, mass spectrometry and a transcriptome-wide tRNA sequencing approach, we observe that TRM1A/TRM1B are responsible for the m22G26 modification of 70% of cytosolic tRNAs in vivo. We use the transcriptome wide tRNAseq approach as well as RNA blot hybridizations to show that RNase P activity is impaired in TRM1A/TRM1B mutants for specific tRNAs, in particular, tRNAs containing a m22G modification at position 26 that are strongly downregulated in TRM1A/TRM1B mutants. Altogether, results indicate that the m22G-adding enzymes TRM1A/TRM1B functionally cooperate with nuclear RNase P in vivo for the early steps of cytosolic tRNA biogenesis. This study shows that the tRNA-modifying enzymes TRM1A/TRM1B are essential to attain the steady-state pool of tRNAs and reveals how they functionally cooperate with RNase P in vivo for the early steps of tRNA biogenesis in Arabidopsis.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"9 12","pages":"2031-2041"},"PeriodicalIF":18.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71524251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-06DOI: 10.1038/s41477-023-01555-1
Yu Su, Tao Feng, Chu-Bin Liu, Haodong Huang, Ya-Ling Wang, Xiaojuan Fu, Mei-Ling Han, Xuanhao Zhang, Xing Huang, Jia-Chen Wu, Tao Song, Hui Shen, Xianpeng Yang, Lin Xu, Shiyou Lü, Dai-Yin Chao
Seed plants overtook ferns to become the dominant plant group during the late Carboniferous, a period in which the climate became colder and dryer1,2. However, the specific innovations driving the success of seed plants are not clear. Here we report that the appearance of suberin lamellae (SL) contributed to the rise of seed plants. We show that the Casparian strip and SL vascular barriers evolved at different times, with the former originating in the most recent common ancestor (MRCA) of vascular plants and the latter in the MRCA of seed plants. Our results further suggest that most of the genes required for suberin formation arose through gene duplication in the MRCA of seed plants. We show that the appearance of the SL in the MRCA of seed plants enhanced drought tolerance through preventing water loss from the stele. We hypothesize that SL provide a decisive selective advantage over ferns in arid environments, resulting in the decline of ferns and the rise of gymnosperms. This study provides insights into the evolutionary success of seed plants and has implications for engineering drought-tolerant crops or fern varieties. This study reports the striking discovery that a water-impermeable barrier known as suberin lamellae was first evolved in the common ancestor of seed plants and contributed to their evolutionary success.
{"title":"The evolutionary innovation of root suberin lamellae contributed to the rise of seed plants","authors":"Yu Su, Tao Feng, Chu-Bin Liu, Haodong Huang, Ya-Ling Wang, Xiaojuan Fu, Mei-Ling Han, Xuanhao Zhang, Xing Huang, Jia-Chen Wu, Tao Song, Hui Shen, Xianpeng Yang, Lin Xu, Shiyou Lü, Dai-Yin Chao","doi":"10.1038/s41477-023-01555-1","DOIUrl":"10.1038/s41477-023-01555-1","url":null,"abstract":"Seed plants overtook ferns to become the dominant plant group during the late Carboniferous, a period in which the climate became colder and dryer1,2. However, the specific innovations driving the success of seed plants are not clear. Here we report that the appearance of suberin lamellae (SL) contributed to the rise of seed plants. We show that the Casparian strip and SL vascular barriers evolved at different times, with the former originating in the most recent common ancestor (MRCA) of vascular plants and the latter in the MRCA of seed plants. Our results further suggest that most of the genes required for suberin formation arose through gene duplication in the MRCA of seed plants. We show that the appearance of the SL in the MRCA of seed plants enhanced drought tolerance through preventing water loss from the stele. We hypothesize that SL provide a decisive selective advantage over ferns in arid environments, resulting in the decline of ferns and the rise of gymnosperms. This study provides insights into the evolutionary success of seed plants and has implications for engineering drought-tolerant crops or fern varieties. This study reports the striking discovery that a water-impermeable barrier known as suberin lamellae was first evolved in the common ancestor of seed plants and contributed to their evolutionary success.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"9 12","pages":"1968-1977"},"PeriodicalIF":18.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号