A novel fluorescent probe with a large Stokes shift for rapidly monitoring hypochlorous acid in living cells, zebrafish, and tobacco seedlings

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED Dyes and Pigments Pub Date : 2025-04-15 DOI:10.1016/j.dyepig.2025.112836

Lin Wang , Ning Zhang , Jian-Yong Wang

{"title":"A novel fluorescent probe with a large Stokes shift for rapidly monitoring hypochlorous acid in living cells, zebrafish, and tobacco seedlings","authors":"Lin Wang , Ning Zhang , Jian-Yong Wang","doi":"10.1016/j.dyepig.2025.112836","DOIUrl":null,"url":null,"abstract":"<div><div>Hypochlorous acid (HClO), as a kind of reactive oxygen species, was produced in the normal aerobic metabolism of organisms. The right amount of HClO can kill invading organisms and fight infection. However, when the amount of HClO is too high, the homeostasis within cells could be disrupted, resulting in irreversible damage to the body. Therefore, HClO plays an important role in normal physiological activities. In this study, a fluorescent probe BTD-TM for monitoring HClO was designed and developed based on benzothiadiazole. When HClO was recognized, the atom S in the thiomorpholine group was oxidized to the sulfoxide structure. Probe BTD-TM showed the advantages of large Stokes shift (120 nm) with low detection limit (LOD = 0.414 μM), high sensitivity, and good stability. Probe BTD-TM was able to achieve the precise detection of endogenous hypochlorous acid with high sensitivity, covering the intracellular homeostasis response induced by n-acetylcysteine and the inflammatory model system triggered by lipopolysaccharide stimulation. At the same time, excellent detection performance was also demonstrated for the tracking of the addition of exogenous hypochlorous acid. In addition, bioimaging showed that BTD-TM could also be used to detect HClO in zebrafish and tobacco seedlings.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"240 ","pages":"Article 112836"},"PeriodicalIF":4.2000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dyes and Pigments","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143720825002062","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Hypochlorous acid (HClO), as a kind of reactive oxygen species, was produced in the normal aerobic metabolism of organisms. The right amount of HClO can kill invading organisms and fight infection. However, when the amount of HClO is too high, the homeostasis within cells could be disrupted, resulting in irreversible damage to the body. Therefore, HClO plays an important role in normal physiological activities. In this study, a fluorescent probe BTD-TM for monitoring HClO was designed and developed based on benzothiadiazole. When HClO was recognized, the atom S in the thiomorpholine group was oxidized to the sulfoxide structure. Probe BTD-TM showed the advantages of large Stokes shift (120 nm) with low detection limit (LOD = 0.414 μM), high sensitivity, and good stability. Probe BTD-TM was able to achieve the precise detection of endogenous hypochlorous acid with high sensitivity, covering the intracellular homeostasis response induced by n-acetylcysteine and the inflammatory model system triggered by lipopolysaccharide stimulation. At the same time, excellent detection performance was also demonstrated for the tracking of the addition of exogenous hypochlorous acid. In addition, bioimaging showed that BTD-TM could also be used to detect HClO in zebrafish and tobacco seedlings.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

一种具有大斯托克斯位移的新型荧光探针，用于快速监测活细胞、斑马鱼和烟草幼苗中的次氯酸

次氯酸（HClO）是生物在正常有氧代谢过程中产生的一种活性氧。适量的HClO可以杀死入侵的生物并抵抗感染。然而，当HClO含量过高时，细胞内的稳态可能会被破坏，对身体造成不可逆的损害。因此，HClO在正常生理活动中起着重要的作用。本研究设计并研制了一种基于苯并噻唑的HClO荧光探针BTD-TM。当HClO被识别时，硫代喹啉基团中的S原子被氧化成亚砜结构。BTD-TM探针具有Stokes位移大（120 nm）、检出限低（LOD = 0.414 μM）、灵敏度高、稳定性好等优点。探针BTD-TM能够以高灵敏度实现内源性次氯酸的精确检测，覆盖了n-乙酰半胱氨酸诱导的细胞内稳态反应和脂多糖刺激引发的炎症模型系统。同时，对外源次氯酸添加的跟踪也表现出优异的检测性能。此外，生物成像显示BTD-TM也可用于检测斑马鱼和烟草幼苗中的HClO。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.