{"title":"Synergistic killing of Escherichia coli K-12 by UV (254 nm) and H2O2.","authors":"A C Leitão, R E Carvalho","doi":"10.1080/09553008814552601","DOIUrl":null,"url":null,"abstract":"<p><p>Prior UV irradiation strongly increased the sensitivity to H2O2 of wild-type E. coli K-12 cells. This synergistic lethal interaction was also observed to a reduced extent in a polA mutant but was absent in uvrA, uvrArecA and xthA mutants. In a recA mutant an antagonist effect was observed. Prior H2O2 treatment did not sensitize the wild-type cells to UV irradiation. Alkaline and neutral sucrose gradient analysis, as well as DNA degradation studies, demonstrated that the synergism is due to the production of DNA double-strand breaks and a block of their repair. The possible mechanism of induction of such lesions is discussed.</p>","PeriodicalId":14254,"journal":{"name":"International journal of radiation biology and related studies in physics, chemistry, and medicine","volume":"53 3","pages":"477-88"},"PeriodicalIF":0.0000,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09553008814552601","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of radiation biology and related studies in physics, chemistry, and medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/09553008814552601","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Prior UV irradiation strongly increased the sensitivity to H2O2 of wild-type E. coli K-12 cells. This synergistic lethal interaction was also observed to a reduced extent in a polA mutant but was absent in uvrA, uvrArecA and xthA mutants. In a recA mutant an antagonist effect was observed. Prior H2O2 treatment did not sensitize the wild-type cells to UV irradiation. Alkaline and neutral sucrose gradient analysis, as well as DNA degradation studies, demonstrated that the synergism is due to the production of DNA double-strand breaks and a block of their repair. The possible mechanism of induction of such lesions is discussed.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
