Serotyping of Campylobacter fetus subsp. fetus and C. fetus subsp. venerealis by passive hemagglutination technique based on soluble autoclaved antigens.

T U Kosunen
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引用次数: 6

Abstract

Antigens for sensitization of sheep erythrocytes were released from C. fetus subsp. fetus and C. fetus subsp. venerealis suspensions by autoclaving in alkaline and neutral saline and by boiling in alkaline but not in neutral saline. Serotyping with rabbit antisera showed the same two serotypes of O-antigens, A and B, as shown by tube agglutination of heated bacteria. All C. fetus subsp. venerealis strains had the A antigen. The C. fetus subsp. fetus strains tested had either the A or B antigen. All ten human isolates tested were of the A type.

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弯曲杆菌胎儿亚种的血清分型。胎儿和C.胎儿亚种基于可溶性高压灭菌抗原的被动血凝技术。
绵羊红细胞致敏抗原从胎儿梭菌中释放出来。胎儿和C.胎儿亚种性病悬浮液在碱性和中性盐水中高压灭菌,在碱性而不是中性盐水中煮沸。用兔抗血清进行血清型分型,经加热菌管凝集可发现o抗原A和B两种相同的血清型。所有C.胎儿亚种。性病株含有A抗原。C.胎儿亚种。胎儿菌株检测有A或B抗原。所有10个人类分离株均为A型。
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