Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1984.TB02791.X
H. Ersgaard, T. Justesen
Ninety-seven strains of clinically isolated Corynebacterium strains, probably identical with Corynebacterium JK, are described especially in regard to growth in relation to different lipid substances. The corynebacteria formed a homogeneous group of strict aerobic slow-growing, catalase-positive, urease-and-nitrate-negative typical coryneform rods. Acid was produced from glucose and maltose. Growth was stimulated in the presence of different lipid substances and lipodependence was suggested by satellite growth only around oleic acid drops on otherwise lipid-depleted agar plates. Generally the isolated corynebacteria were resistant to clinically achievable concentrations of penicillins, cephalosporines and aminoglucosides but uniformly sensitive to vancomycin and rifamycin.
{"title":"Multiresistant lipophilic corynebacteria from clinical specimens. Biochemical reactions and antimicrobial agents susceptibility.","authors":"H. Ersgaard, T. Justesen","doi":"10.1111/J.1699-0463.1984.TB02791.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1984.TB02791.X","url":null,"abstract":"Ninety-seven strains of clinically isolated Corynebacterium strains, probably identical with Corynebacterium JK, are described especially in regard to growth in relation to different lipid substances. The corynebacteria formed a homogeneous group of strict aerobic slow-growing, catalase-positive, urease-and-nitrate-negative typical coryneform rods. Acid was produced from glucose and maltose. Growth was stimulated in the presence of different lipid substances and lipodependence was suggested by satellite growth only around oleic acid drops on otherwise lipid-depleted agar plates. Generally the isolated corynebacteria were resistant to clinically achievable concentrations of penicillins, cephalosporines and aminoglucosides but uniformly sensitive to vancomycin and rifamycin.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"75 1","pages":"39-43"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83601949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/j.1699-0463.1982.tb00079.x
E. Gutschik
The synergistic action of penicillin and streptomycin was investigated on animals with experimental endocarditis, using the previously described model and four different strains of Streptococcus faecalis. Two strains represented a moderate and two strains a high level of resistance to streptomycin. The purpose was to determine the effect of the combined penicillin and streptomycin treatment, since previous in vitro investigations showed that strains highly resistant to streptomycin were also resistant to combination of the drugs. Antibiotic treatment of the animals was carried out for 28 days, followed by a period of four weeks observation. The treatment resulted in a demonstrable effect against infection caused by the least streptomycin-resistant strain, but was completely ineffective in rabbits infected with the strain homogeneously resistant to 8000 micrograms/ml streptomycin. The synergistic effect of penicillin and streptomycin towards strains within a range of streptomycin resistance of 2000-8000 micrograms/ml could be predicted by the IC50 test, but not by MIC or the in vitro killing curve test for synergism. Besides the therapeutic results, this report also considers the following features: pathoanatomic and physiologic processes related to the number of viable bacteria in the endocardial vegetations; the influence of the proteolytic capacity of infecting strains; the development of congestive heart failure.
{"title":"Experimental endocarditis in rabbits. 6. Results of long-term combined therapy of Streptococcus faecalis endocarditis with penicillin and streptomycin.","authors":"E. Gutschik","doi":"10.1111/j.1699-0463.1982.tb00079.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1982.tb00079.x","url":null,"abstract":"The synergistic action of penicillin and streptomycin was investigated on animals with experimental endocarditis, using the previously described model and four different strains of Streptococcus faecalis. Two strains represented a moderate and two strains a high level of resistance to streptomycin. The purpose was to determine the effect of the combined penicillin and streptomycin treatment, since previous in vitro investigations showed that strains highly resistant to streptomycin were also resistant to combination of the drugs. Antibiotic treatment of the animals was carried out for 28 days, followed by a period of four weeks observation. The treatment resulted in a demonstrable effect against infection caused by the least streptomycin-resistant strain, but was completely ineffective in rabbits infected with the strain homogeneously resistant to 8000 micrograms/ml streptomycin. The synergistic effect of penicillin and streptomycin towards strains within a range of streptomycin resistance of 2000-8000 micrograms/ml could be predicted by the IC50 test, but not by MIC or the in vitro killing curve test for synergism. Besides the therapeutic results, this report also considers the following features: pathoanatomic and physiologic processes related to the number of viable bacteria in the endocardial vegetations; the influence of the proteolytic capacity of infecting strains; the development of congestive heart failure.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"61 1","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88957848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1982.TB00097.X
L. Odum, I. Lind
Sixty-seven out of 88 Neisseria gonorrhoeae strains isolated from test-of-cure (TOC) specimens during a five-months' period were included in the study. For 62 patients sufficient information was obtained in order to distinguish between relapse (34 ptt) and re-infection (28 ptt). For comparison with strains from these two groups of patients, 63 urogenital and 21 pharyngeal gonococcal strains isolated during the same period of time were randomly selected. The distributions according to susceptibility to penicillin for TOC strains and control strains corresponded to those found for the total number of TOC strains (275) and other strains (3,345) tested in 1979, respectively. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent bactericidal activity of normal human serum. However, gonococcal strains less susceptible to penicillin in vitro (MIC values within the range 0.1-2.0 microgram/ml) were significantly more sensitive to the complement-dependent activity of normal human serum (P less than 0.01) than strains fully susceptible to penicillin (MIC less than 0.01 microgram/ml.) Penicillin-resistant strains (MIC greater than 2.0 microgram/ml) did not differ from strains susceptible to less than 0.1 microgram penicillin/ml and were slightly more serum-resistant than the less susceptible strains (P less than 0.05). No difference in serum-sensitivity of urogenital and pharyngeal isolates could be demonstrated. The level of bactericidal activity of homologous convalescent serum was unrelated to the presence of antibodies either to gonococcal pili or crude gonococcal antigen preparations. The sensitivity to normal human serum of a certain strain was not correlated with sensitivity to homologous convalescent serum.
{"title":"Studies on Neisseria gonorrhoeae strains from test-of-cure specimens. Correlation between the in vitro susceptibility to penicillin and the sensitivity to the complement-dependent bactericidal activity of normal and convalescent human serum.","authors":"L. Odum, I. Lind","doi":"10.1111/J.1699-0463.1982.TB00097.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1982.TB00097.X","url":null,"abstract":"Sixty-seven out of 88 Neisseria gonorrhoeae strains isolated from test-of-cure (TOC) specimens during a five-months' period were included in the study. For 62 patients sufficient information was obtained in order to distinguish between relapse (34 ptt) and re-infection (28 ptt). For comparison with strains from these two groups of patients, 63 urogenital and 21 pharyngeal gonococcal strains isolated during the same period of time were randomly selected. The distributions according to susceptibility to penicillin for TOC strains and control strains corresponded to those found for the total number of TOC strains (275) and other strains (3,345) tested in 1979, respectively. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent. The TOC strains did not differ from the control strains in sensitivity to the complement-dependent bactericidal activity of normal human serum. However, gonococcal strains less susceptible to penicillin in vitro (MIC values within the range 0.1-2.0 microgram/ml) were significantly more sensitive to the complement-dependent activity of normal human serum (P less than 0.01) than strains fully susceptible to penicillin (MIC less than 0.01 microgram/ml.) Penicillin-resistant strains (MIC greater than 2.0 microgram/ml) did not differ from strains susceptible to less than 0.1 microgram penicillin/ml and were slightly more serum-resistant than the less susceptible strains (P less than 0.05). No difference in serum-sensitivity of urogenital and pharyngeal isolates could be demonstrated. The level of bactericidal activity of homologous convalescent serum was unrelated to the presence of antibodies either to gonococcal pili or crude gonococcal antigen preparations. The sensitivity to normal human serum of a certain strain was not correlated with sensitivity to homologous convalescent serum.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"11 19 1","pages":"145-52"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90221992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1986.TB03020.X
P. Kjaeldgaard, B. Nissen, N. Lange, H. Laursen
Minibact, a new system for four-hour identification of Enterobacteriaceae, combined with a computer identification system, was compared with Micro-ID and API 20E in testing 110 strains of Enterobacteriaceae. Minibact gave identification rates of 96.4% at species level and 96.4% at genus level; the corresponding values for Micro-ID were 87.3% and 91.8%, and for API 20E 91.8% and 94.5%. In conclusion, Minibact combined with a computer identification program gave high identification rates fully comparable to those of Micro-ID and API 20E, and the system might be an alternative to conventional identification systems in clinical microbiological departments.
{"title":"Evaluation of Minibact, a new system for rapid identification of Enterobacteriaceae. Comparison of Minibact, Micro-ID and API 20E with a conventional method as reference.","authors":"P. Kjaeldgaard, B. Nissen, N. Lange, H. Laursen","doi":"10.1111/J.1699-0463.1986.TB03020.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1986.TB03020.X","url":null,"abstract":"Minibact, a new system for four-hour identification of Enterobacteriaceae, combined with a computer identification system, was compared with Micro-ID and API 20E in testing 110 strains of Enterobacteriaceae. Minibact gave identification rates of 96.4% at species level and 96.4% at genus level; the corresponding values for Micro-ID were 87.3% and 91.8%, and for API 20E 91.8% and 94.5%. In conclusion, Minibact combined with a computer identification program gave high identification rates fully comparable to those of Micro-ID and API 20E, and the system might be an alternative to conventional identification systems in clinical microbiological departments.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"51 1","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76549071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1986.TB03015.X
Peder Bo Nielsen, Pierre Goyot, Madeleine Mojon
An assessment of the Gram, the Methylene blue, and the Ziehl-Neelsen procedures in diagnosing Pneumocystis carinii has been performed. By using heat and methanol as fixatives and phase-contrast microscopy we found the procedures valuable in detecting the pneumocysts, especially in the Gram staining. The diagnosis should, however, be confirmed by a re-staining with a specific staining, viz. the toluidine blue 0 or the silver impregnation a.m. Gomori-Grocott. It is additionally shown that the staining a.m. Gomori-Grocott is dependent on the density of the preparation.
{"title":"The microscopic diagnosis of Pneumocystis carinii. An evaluation of the gram, the methylene blue, and the Ziehl-Neelsen procedures.","authors":"Peder Bo Nielsen, Pierre Goyot, Madeleine Mojon","doi":"10.1111/J.1699-0463.1986.TB03015.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1986.TB03015.X","url":null,"abstract":"An assessment of the Gram, the Methylene blue, and the Ziehl-Neelsen procedures in diagnosing Pneumocystis carinii has been performed. By using heat and methanol as fixatives and phase-contrast microscopy we found the procedures valuable in detecting the pneumocysts, especially in the Gram staining. The diagnosis should, however, be confirmed by a re-staining with a specific staining, viz. the toluidine blue 0 or the silver impregnation a.m. Gomori-Grocott. It is additionally shown that the staining a.m. Gomori-Grocott is dependent on the density of the preparation.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"18 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73740832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1982.TB00139.X
G. Bukholm, J. Lassen
The invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family Enterobacteriaceae have been tested using the Serény test and HEp-2 cell monolayers examined by a combined light optical method. All the presumptive enteropathogenic strains of Shigella dysenteriae, S. boydii, S. flexneri, S. sonnei and Salmonella typhimurium showed in vitro invasiveness in the HEp-2 cell culture test. Fourteen presumptive non-enteropathogenic strains of Escherichia coli showed no invasiveness in either of the two test systems. Two strains of S. flexneri and all the 6 strains of S. typhimurium gave a negative result in the Serény test although they were invasive in HEp-2 cell cultures. Otherwise there were correlative results between the cell monolayer test and the Serény test. In the cell monolayer test the different species of enteropathogenic bacteria showed considerable variation in invasive potential.
{"title":"Bacterial adhesiveness and invasiveness in cell culture monolayer. 2. In vitro invasiveness of 45 strains belonging to the family Enterobacteriaceae.","authors":"G. Bukholm, J. Lassen","doi":"10.1111/J.1699-0463.1982.TB00139.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1982.TB00139.X","url":null,"abstract":"The invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family Enterobacteriaceae have been tested using the Serény test and HEp-2 cell monolayers examined by a combined light optical method. All the presumptive enteropathogenic strains of Shigella dysenteriae, S. boydii, S. flexneri, S. sonnei and Salmonella typhimurium showed in vitro invasiveness in the HEp-2 cell culture test. Fourteen presumptive non-enteropathogenic strains of Escherichia coli showed no invasiveness in either of the two test systems. Two strains of S. flexneri and all the 6 strains of S. typhimurium gave a negative result in the Serény test although they were invasive in HEp-2 cell cultures. Otherwise there were correlative results between the cell monolayer test and the Serény test. In the cell monolayer test the different species of enteropathogenic bacteria showed considerable variation in invasive potential.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"14 1","pages":"409-13"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87055742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1984.TB02795.X
L. E. Ravdonikas, P. Christensen, L. Burova, K. Grabovskaya, L. Björck, C. Schalén, M. Svensson, A. Totolian
Recently, we reported that conjugal transfer of plasmid pERL1, determining i.a. erythromycin resistance (Emr), into group A streptococci could trigger the expression of anti-phagocytic activity, adhesiveness, opacity factor and capacity to bind immunoglobulin Fc-parts and beta 2-microglobulin. In the present study, ethidium bromide treatment of Emr transconjugants allowed the selection of "cured", erythromycin sensitive (Ems) mutants. This procedure did not affect the expression of the abovementioned characteristics. However, when plasmid pERL1 was again transferred to two such mutants, the "secondary", Emr transconjugants obtained showed lack of each of these properties. Our experiments thus demonstrated a "switch-on" as well as a "switch-off" effect, exerted by the same plasmid, pERL1, on some major pathogenic properties of group A streptococci.
{"title":"The genetic control of virulence in group A streptococci. III. Plasmid-induced \"switch-off\"--effect on some pathogenic properties.","authors":"L. E. Ravdonikas, P. Christensen, L. Burova, K. Grabovskaya, L. Björck, C. Schalén, M. Svensson, A. Totolian","doi":"10.1111/J.1699-0463.1984.TB02795.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1984.TB02795.X","url":null,"abstract":"Recently, we reported that conjugal transfer of plasmid pERL1, determining i.a. erythromycin resistance (Emr), into group A streptococci could trigger the expression of anti-phagocytic activity, adhesiveness, opacity factor and capacity to bind immunoglobulin Fc-parts and beta 2-microglobulin. In the present study, ethidium bromide treatment of Emr transconjugants allowed the selection of \"cured\", erythromycin sensitive (Ems) mutants. This procedure did not affect the expression of the abovementioned characteristics. However, when plasmid pERL1 was again transferred to two such mutants, the \"secondary\", Emr transconjugants obtained showed lack of each of these properties. Our experiments thus demonstrated a \"switch-on\" as well as a \"switch-off\" effect, exerted by the same plasmid, pERL1, on some major pathogenic properties of group A streptococci.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"206 1","pages":"65-9"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73136122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1983.TB00046.X
B. Hansen
A total of 965 strains of Micrococcaceae isolated from 200 patients, personnel, and air in a department of cardiac surgery were classified by means of Baird-Parker's scheme. The majority of strains were identified as Staphylococcus epidermidis (S. epidermidis) biotype 1, but S. epidermidis biotype 4 accounted for c. 25% of isolates from patients post-operatively. Pre-operative isolates were generally sensitive to most antibiotics tested while post-operative strains of coagulase-negative Micrococcaceae from patients and isolates from personnel and air were frequently multiply-resistant. Strains of Staphylococcus aureus (S. aureus) were sensitive or resistant only to penicillin. More patients were colonized with coagulase-negative Micrococcaceae after operation than at admission to the hospital (p less than 0.001), while the frequency of S. aureus carriers was the same before and after operation and equal to the frequency found earlier. The frequency of S. aureus carriers among the personnel, however, was lower than reported earlier (10%). Multiply-resistant strains of S. epidermidis seem to have replaced resistant strains of S. aureus as the predominant hospital saprophyte among Micrococcaceae.
{"title":"Investigation of Micrococcaceae in a department of cardiac surgery. Biochemical characterization and sensitivity patterns of strains isolated from patients, staff, and air.","authors":"B. Hansen","doi":"10.1111/J.1699-0463.1983.TB00046.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1983.TB00046.X","url":null,"abstract":"A total of 965 strains of Micrococcaceae isolated from 200 patients, personnel, and air in a department of cardiac surgery were classified by means of Baird-Parker's scheme. The majority of strains were identified as Staphylococcus epidermidis (S. epidermidis) biotype 1, but S. epidermidis biotype 4 accounted for c. 25% of isolates from patients post-operatively. Pre-operative isolates were generally sensitive to most antibiotics tested while post-operative strains of coagulase-negative Micrococcaceae from patients and isolates from personnel and air were frequently multiply-resistant. Strains of Staphylococcus aureus (S. aureus) were sensitive or resistant only to penicillin. More patients were colonized with coagulase-negative Micrococcaceae after operation than at admission to the hospital (p less than 0.001), while the frequency of S. aureus carriers was the same before and after operation and equal to the frequency found earlier. The frequency of S. aureus carriers among the personnel, however, was lower than reported earlier (10%). Multiply-resistant strains of S. epidermidis seem to have replaced resistant strains of S. aureus as the predominant hospital saprophyte among Micrococcaceae.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"13 1","pages":"273-8"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75977696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1983.TB00007.X
V. Myhrvold, J. Jonsen, B. Mørland
Sheep erythrocytes opsonized with IgG or C3b were frozen in various cryoprotective agents, thawed, and compared to corresponding unfrozen erythrocytes exposed to the cryoprotectants and to unfrozen erythrocytes not exposed to the cryoprotectants (controls) as test particles in macrophage attachment and phagocytosis assays. Fc-receptor-mediated attachment and phagocytosis were not influenced by the use of any cryoprotective agent tested or by freezing the erythrocytes. This was also the case with C3b-receptor-mediated attachment. Phagocytosis via this receptor was negligible in normal macrophages, but tended to be slightly more effective when the test particles had been treated with cryoprotective agents. In vitro stimulation of the macrophages with Escherichia coli endotoxin, however, triggered the capacity to internalize treated and untreated erythrocytes equally.
{"title":"The use of frozen erythrocytes in macrophage studies. 2. Attachment and phagocytosis mediated by the two immunological receptors of the macrophages.","authors":"V. Myhrvold, J. Jonsen, B. Mørland","doi":"10.1111/J.1699-0463.1983.TB00007.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1983.TB00007.X","url":null,"abstract":"Sheep erythrocytes opsonized with IgG or C3b were frozen in various cryoprotective agents, thawed, and compared to corresponding unfrozen erythrocytes exposed to the cryoprotectants and to unfrozen erythrocytes not exposed to the cryoprotectants (controls) as test particles in macrophage attachment and phagocytosis assays. Fc-receptor-mediated attachment and phagocytosis were not influenced by the use of any cryoprotective agent tested or by freezing the erythrocytes. This was also the case with C3b-receptor-mediated attachment. Phagocytosis via this receptor was negligible in normal macrophages, but tended to be slightly more effective when the test particles had been treated with cryoprotective agents. In vitro stimulation of the macrophages with Escherichia coli endotoxin, however, triggered the capacity to internalize treated and untreated erythrocytes equally.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"13 1","pages":"43-7"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78805102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1983.TB00015.X
B. Adnegard, T. Horstad
nucleatum lipopolysaccharides (LPS) of chemotype V were examined for cross-reactivity by indirect haemagglutination in non-absorbed and absorbed antisera. At least three O-antigenic specificities were detected in LPS of five strains. There was a slight cross-reactivity between the LPS of chemotype V and those of chemotype II.
{"title":"O-antigenic corss-reactivity in Fusobacterium nucleatum: chemotype V lipopolysaccharides.","authors":"B. Adnegard, T. Horstad","doi":"10.1111/J.1699-0463.1983.TB00015.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1983.TB00015.X","url":null,"abstract":"nucleatum lipopolysaccharides (LPS) of chemotype V were examined for cross-reactivity by indirect haemagglutination in non-absorbed and absorbed antisera. At least three O-antigenic specificities were detected in LPS of five strains. There was a slight cross-reactivity between the LPS of chemotype V and those of chemotype II.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"30 1","pages":"93-5"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84403660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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