{"title":"Same-day confirmation of Staphylococcus aureus bacteraemia by a thermonuclease test.","authors":"K Bergh, J A Maeland","doi":"10.1111/j.1699-0463.1986.tb03055.x","DOIUrl":null,"url":null,"abstract":"<p><p>A test to detect thermostable DNase activity (TDNase test) was evaluated for rapid confirmation of S. aureus bacteraemia, using toluidine blue O DNA agar plates for the testing. A total of 226 blood cultures which grew bacteria were examined. S. aureus was identified in 76 of the cultures. All of the S. aureus isolates showed positive TDNase test after 2 h of incubation of the test plates. The remaining isolates examined, most of them coagulase-negative staphylococci, showed a negative test. The TDNase test enables reliable same-day confirmation of S. aureus bacteraemia.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 4","pages":"291-2"},"PeriodicalIF":0.0000,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03055.x","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1699-0463.1986.tb03055.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
A test to detect thermostable DNase activity (TDNase test) was evaluated for rapid confirmation of S. aureus bacteraemia, using toluidine blue O DNA agar plates for the testing. A total of 226 blood cultures which grew bacteria were examined. S. aureus was identified in 76 of the cultures. All of the S. aureus isolates showed positive TDNase test after 2 h of incubation of the test plates. The remaining isolates examined, most of them coagulase-negative staphylococci, showed a negative test. The TDNase test enables reliable same-day confirmation of S. aureus bacteraemia.
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