[Cell division and energy dependent metabolic power of Ehrlich-ascites-tumor cells under aerobic and anaerobic conditions].

Abstract

Interrelations between energy metabolism and cell division were studied with permanent in vitro cultured Ehrlich-ascites-tumor-cells during the second passage in vitro. The following parameters were estimated under aerobic and anaerobic conditions and in the presence of dinitrophenol: DNA- and RNA-synthesis measured by the incorporation of 14C-thymidine and 14C-uridine; rate of glycolysis measured by lactate production; permeation of 14C-2-aminoisobutyric acid; ATP level of the cells; cell division and number of dead cells. The following results were obtained: Cell division and thymidine incorporation are obligate oxygen dependent processes. It was not possible to decide, whether respiration alone can maintain cell propagation. Respiration alone and glycolysis alone cannot maintain a normal level of ATP. Only an intact respiration can maintain the penetration of 2-aminoiso-butyric acid. Glycolysis alone and respiration alone can supply the energy for the incorporation of uridine. The incorporation of uridine is also influenced by oxygen dependent reactions not related to energy production. The number of dead cells does not rise significantly under anaerobic conditions.