Secondary structure of intraphage DNA of coliphage T-7 was studied by spectrophotometry and electron microscopy. T-7 DNA in vivo showed 7% hyperchromicity, on the average, compared to DNA released by heating the phage 30 min at 55 - 60 °C. The intraphage DNA when melted by heating the phage for 13 min at 95° and then rapidly chilled, was found to be defficient in hyperchromic rise by about 10% compared to isolated T-7-DNA treated under similar conditions. Electron micrographs of T-7-DNA released by osmotic shock revealed that the incompletely released molecules showing a pattern possessed denatured regions in the form of single-stranded loops. Phenol extracted T-7-DNA molecules as well as T-7 genomes completely released by osmotic shock did not show any pattern and possessed no denatured zones. These results helped to infer that intraphage T-7-DNA is partially denatured.
{"title":"Studies on the secondary structure of intraphage T-7 DNA.","authors":"R K Sinha, D N Misra","doi":"10.1515/znb-1971-1218","DOIUrl":"https://doi.org/10.1515/znb-1971-1218","url":null,"abstract":"Secondary structure of intraphage DNA of coliphage T-7 was studied by spectrophotometry and electron microscopy. T-7 DNA in vivo showed 7% hyperchromicity, on the average, compared to DNA released by heating the phage 30 min at 55 - 60 °C. The intraphage DNA when melted by heating the phage for 13 min at 95° and then rapidly chilled, was found to be defficient in hyperchromic rise by about 10% compared to isolated T-7-DNA treated under similar conditions. Electron micrographs of T-7-DNA released by osmotic shock revealed that the incompletely released molecules showing a pattern possessed denatured regions in the form of single-stranded loops. Phenol extracted T-7-DNA molecules as well as T-7 genomes completely released by osmotic shock did not show any pattern and possessed no denatured zones. These results helped to infer that intraphage T-7-DNA is partially denatured.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1288-91"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znb-1971-1218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The significance of metabolic energy and the ion pump for the receptor potential of the crayfish photoreceptor cell.","authors":"H Stieve, H Bollmann-Fischer, B Braun","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1311-21"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
[ 1 9 5 9 ] . The nature of the restricted rotation in dialkylaminosulphinyl chlorides (3) is under investigation. Dimethylaminosulphinyl chloride (3 a) was prepared from O-ethyl ./V,iV-dimethylamidosulphite and thionvl chloride12; b.p. 30°/0.2 mmHg, ng 1.4960 (I.e.13 43 — 45°/0.3 mmHg). The chlorides (3 b) and (3 c) were synthesized directly from the corresponding amines and thionyl chloride 13; 3 b: b.p. 62°/0.6mmHg, tid 1.5165; 3 c: b.p. 75 — 76°/0.4 mmHg, semisolid compound at room temperature.
{"title":"[Purification of a pseudo-cholinesterase from the haemolymph of Helix pomatia].","authors":"R Voigtmann, G Uhlenbruck","doi":"10.1515/znb-1971-1237","DOIUrl":"https://doi.org/10.1515/znb-1971-1237","url":null,"abstract":"[ 1 9 5 9 ] . The nature of the restricted rotation in dialkylaminosulphinyl chlorides (3) is under investigation. Dimethylaminosulphinyl chloride (3 a) was prepared from O-ethyl ./V,iV-dimethylamidosulphite and thionvl chloride12; b.p. 30°/0.2 mmHg, ng 1.4960 (I.e.13 43 — 45°/0.3 mmHg). The chlorides (3 b) and (3 c) were synthesized directly from the corresponding amines and thionyl chloride 13; 3 b: b.p. 62°/0.6mmHg, tid 1.5165; 3 c: b.p. 75 — 76°/0.4 mmHg, semisolid compound at room temperature.","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1374-5"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Paraproteins and normal immunoglobulins of the lambda type: immunochemical differentiation and classification].","authors":"F W Tischendorf","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1292-302"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15224753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunosuppressive action in vitro by a bovine spleen extract.","authors":"V H Morales, E Garcia-Giralt","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1339-40"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Comparative study on the uropygial gland lipids of the greenfinch (Carduelis chloris), bullfinch (Pyrrhula pyrrhula) and the linnet (Carduelis cannabina)].","authors":"J Jacob, A Zeman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1352-6"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Increased activity of turkey insulin in various test systems].","authors":"J Hahn, O Martini","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23706,"journal":{"name":"Zeitschrift fur Naturforschung. Teil B, Chemie, Biochemie, Biophysik, Biologie und verwandte Gebiete","volume":"26 12","pages":"1378"},"PeriodicalIF":0.0,"publicationDate":"1971-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15503192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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