Theoretical and Practical Studies on Effects of External Electrostatic Electric Field on Nucleation and Growth Kinetics of Protein Crystals

IF 4.5 2区材料科学 Q1 CRYSTALLOGRAPHY Progress in Crystal Growth and Characterization of Materials Pub Date : 2022-08-01 DOI:10.1016/j.pcrysgrow.2022.100568

Haruhiko Koizumi , Satoshi Uda

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引用次数: 2

Abstract

The crystallization technique where an electric field is applied is an extremely powerful tool to control the crystallization processes of various materials. In particular, the method with application of an external electrostatic electric field can have a significant effect on the phase equilibrium of the liquid and solid phases. This review demonstrates that the crystallization processes of proteins are significantly impacted by the application of an external electrostatic electric field: (1) Control of both the increase and decrease in the nucleation rate can be achieved by changing the applied frequency of the external electrostatic electric field. (2) The effect of the external electrostatic electric field on the nucleation rate can be controlled by regulating the thickness of the electric double layer (EDL) formed at the interface. (3) The quality of the grown crystals can be improved by an increase in the step free energy under application of an external electrostatic electric field at 1 MHz. The effect of the external electrostatic electric field on nucleation and growth kinetics during crystal growth of proteins is also discussed based on a thermodynamic perspective.

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外加静电场对蛋白质晶体成核和生长动力学影响的理论与实践研究

施加电场的结晶技术是控制各种材料结晶过程的一种极为有力的工具。特别是外加静电场的方法对液固两相的相平衡有显著的影响。本文的研究表明，外加静电场对蛋白质的结晶过程有显著的影响:(1)通过改变外加静电场的施加频率可以控制成核速率的增加和减少。(2)外部静电场对成核速率的影响可以通过调节界面处形成的双电层(EDL)的厚度来控制。(3)在1 MHz的外加静电场下，增加阶跃自由能可以提高生长晶体的质量。本文还从热力学角度讨论了外加静电场对蛋白质晶体生长过程中成核和生长动力学的影响。

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来源期刊

Progress in Crystal Growth and Characterization of Materials 工程技术-材料科学：表征与测试

CiteScore

8.80

自引率

2.00%

发文量

审稿时长

1 day

期刊介绍： Materials especially crystalline materials provide the foundation of our modern technologically driven world. The domination of materials is achieved through detailed scientific research. Advances in the techniques of growing and assessing ever more perfect crystals of a wide range of materials lie at the roots of much of today''s advanced technology. The evolution and development of crystalline materials involves research by dedicated scientists in academia as well as industry involving a broad field of disciplines including biology, chemistry, physics, material sciences and engineering. Crucially important applications in information technology, photonics, energy storage and harvesting, environmental protection, medicine and food production require a deep understanding of and control of crystal growth. This can involve suitable growth methods and material characterization from the bulk down to the nano-scale.

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