Controls on the hydrogen isotope composition of tetraether lipids in an autotrophic ammonia-oxidizing marine archaeon

Abstract

The stable hydrogen isotope composition of persistent biomolecules is used as a palaeohydrological proxy. While much previous work has focused on plant leaf wax-derived n-alkanes, the potential of prokaryotic lipid biomarkers as carriers of H isotope signatures remains underexplored, particularly in the Archaea. Here we investigated H isotope distributions in the membrane lipids of the ammonia-oxidizing chemoautotroph Nitrosopumilus maritimus strain SCM1. Hydrogen isotope ratios were measured on the cleaved biphytane chains of tetraether membrane lipids extracted from steady-state continuous cultures cultivated at slow, medium, and fast growth rates. In contrast to recent work on bacterial fatty acids, where the direction and magnitude of isotopic fractionation varies widely (ca. 600‰ range) as a function of central C and energy metabolism, archaeal biphytane data in the present work are relatively invariant. The weighted average ²H/¹H fractionation values relative to growth water (²ε_L/W) ranged from –272 to –260‰, despite a three-fold difference in doubling times (30.8–92.5 hr), yielding an average growth-rate effect <0.2‰ hr⁻¹. These ²ε_L/W values are more negative than most heterotrophic microbial lipid H isotope measurements in the literature, and are on par with those from other autotrophic archaea, as well as with phytol from photoautotrophic algae. N. maritimus values of ²ε_L/W also varied systematically with the number of internal rings (cyclopentyl + cyclohexyl), increasing for each additional ring by 6.4 ± 2.7‰. Using an isotope flux-balance model in tandem with a comprehensive analysis of the sources of H in archaeal lipid biosynthesis, we use this observation to estimate the kinetic isotope effects (KIEs) of H incorporation from water; from reducing cofactors such as flavins and NADPH, and for the transhydrogenation reaction(s) that convert the electron-donor derived NADH into these cofactors. Consistent with prior studies on bacteria and plants, our results indicate the KIEs of reducing cofactors in archaea are highly fractionating, while those involving exchange of water protons are less so. When combined with the observation of minimal growth-rate sensitivity, our results suggest biphytanes of autotrophic 3HP/4HB utilizing Nitrososphaerota (a.k.a. Thaumarchaeota) may be offset from their growth waters by a nearly constant ²ε_L/W value. Together with the ring effect, this implies that all biphytanes originating from a common source should have a predictable ordering of their isotope ratios with respect to biphytane ring number, allowing precise reconstruction of the original δ²H value of the environmental water. Collectively, these patterns indicate archaeal biphytanes have potential as paleo-hydrological proxies, either as a complement or an alternative to leaf wax n-alkanes.