Decoration of Bi2O3NPs-AgNPs-ErGO as a first electrochemical nanosensor for sensitive determination of nelarabine in pharmaceutical dosage form and human serum samples

IF 4.5 3区 化学 Q1 Chemical Engineering Journal of Electroanalytical Chemistry Pub Date : 2023-09-01 DOI:10.1016/j.jelechem.2023.117651
Md. Zahirul Kabir , Cem Erkmen , Sevinc Kurbanoglu , Gözde Aydogdu Tig , Bengi Uslu
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Abstract

In view of the unquestionable necessity for rapid and accurate analysis of drug molecules to monitor patient overdose, numerous nanosensor-based technologies have been developed in addition to quality control of pharmaceutical manufacture and drug administration. In this regard, sensitive detection of a potent anticancer agent, nelarabine (NEL) was examined at the bare and modified glassy carbon electrode (GCE) with the help of differential pulse (DP) and cyclic voltammetry (CV) techniques. A unique and highly effective nanosensor was developed using a combination of bismuth (III) oxide nanoparticles (Bi2O3NPs), silver nanoparticles (AgNPs), and electrochemically reduced graphene oxide (ErGO) onto the GCE surface. The modified Bi2O3NPs-AgNPs-ErGO/GCE was characterized by scanning electron microscopy (SEM), CV, and electrochemical impedance spectroscopy (EIS) investigations. Influences of various parameters viz., loading of Bi2O3NPs, AgNPs, and GO on the modified GCE, electrolyte pH (PBS 7.0), accumulation potential (–0.2 V), and time (60 s), and scan rate (50 mV s−1) were optimized for NEL response. An enhancement in the current responses toward the oxidation of NEL was observed with the Bi2O3NPs-AgNPs-ErGO/GCE compared to that noticed with bare GCE. The modified GCE affirmed high sensitivity, low limit of detection (LOD), excellent reproducibility, repeatability, and storage stability that clearly indicated the effective accuracy of the developed nanosensor. The linear behavior in the concentration range was found to be 0.02–1.0 µM, with LOD values of 0.003 nM in PBS 7.0 and 0.065 nM in serum samples. The electrochemical mechanism of NEL at the bare and modified GCEs were revealed as diffusion-controlled and adsorption-controlled mechanism processes, respectively. The linear calibration curves at both the bare and modified GCEs were noticed for increasing NEL concentrations, as constructed from the DPV measurements. Applications of the Bi2O3NPs-AgNPs-ErGO/GCE for NEL detection in pharmaceutical dosage form and human serum sample showed well-accepted recovery results of 98–99 %. The effect of interfering agents was checked on the selectivity of the developed method, and the modified electrode was found to be selective toward NEL in the presence of these interfering agents.

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修饰Bi2O3NPs-AgNPs-ErGO作为首个电化学纳米传感器用于药物剂型和人血清样品中奈拉宾的灵敏测定
鉴于对药物分子进行快速和准确的分析以监测患者服药过量的必要性,除了对药品生产和药物管理进行质量控制外,还开发了许多基于纳米传感器的技术。在这方面,利用差分脉冲(DP)和循环伏安法(CV)技术,研究了一种有效的抗癌药物奈拉宾(NEL)在裸露和修饰的玻璃碳电极(GCE)上的敏感检测。将铋(III)氧化物纳米粒子(Bi2O3NPs)、银纳米粒子(AgNPs)和电化学还原氧化石墨烯(ErGO)结合在GCE表面,开发了一种独特且高效的纳米传感器。通过扫描电镜(SEM)、CV和电化学阻抗谱(EIS)对改性Bi2O3NPs-AgNPs-ErGO/GCE进行了表征。优化了Bi2O3NPs、AgNPs和GO负载等参数对改性GCE、电解液pH (PBS 7.0)、积累电位(-0.2 V)、时间(60 s)和扫描速率(50 mV s−1)的影响。与纯GCE相比,Bi2O3NPs-AgNPs-ErGO/GCE对NEL氧化的电流响应增强。改进后的GCE具有高灵敏度、低检出限(LOD)、良好的重现性、可重复性和存储稳定性,表明所开发的纳米传感器具有有效的准确性。在浓度范围内线性行为为0.02 ~ 1.0µM,在PBS 7.0中LOD值为0.003 nM,在血清样品中LOD值为0.065 nM。结果表明,NEL在裸gce和改性gce上的电化学机制分别为扩散控制机制和吸附控制机制。根据DPV测量结果,在裸gce和修饰gce处的线性校准曲线都注意到NEL浓度的增加。应用Bi2O3NPs-AgNPs-ErGO/GCE检测药物剂型和人血清样品的NEL,回收率为98 ~ 99%。研究了干扰剂对该方法选择性的影响,发现在干扰剂存在的情况下,修饰电极对NEL具有选择性。
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来源期刊
Journal of Electroanalytical Chemistry
Journal of Electroanalytical Chemistry Chemical Engineering-General Chemical Engineering
CiteScore
7.50
自引率
6.70%
发文量
912
审稿时长
>12 weeks
期刊介绍: The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied. Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.
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