Vitrification as a prospect for cryopreservation of tissue-engineered constructs

IF 12.8 1区 医学 Q1 ENGINEERING, BIOMEDICAL Biomaterials Pub Date : 2007-03-01 DOI:10.1016/j.biomaterials.2006.11.047
L.L. Kuleshova , S.S. Gouk , D.W. Hutmacher
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引用次数: 90

Abstract

Cryopreservation plays a significant function in tissue banking and will presume yet larger value when more and more tissue-engineered products will routinely enter the clinical arena. The most common concept underlying tissue engineering is to combine a scaffold (cellular solids) or matrix (hydrogels) with living cells to form a tissue-engineered construct (TEC) to promote the repair and regeneration of tissues. The scaffold and matrix are expected to support cell colonization, migration, growth and differentiation, and to guide the development of the required tissue. The promises of tissue engineering, however, depend on the ability to physically distribute the products to patients in need. For this reason, the ability to cryogenically preserve not only cells, but also TECs, and one day even whole laboratory-produced organs, may be indispensable. Cryopreservation can be achieved by conventional freezing and vitrification (ice-free cryopreservation). In this publication we try to define the needs versus the desires of vitrifying TECs, with particular emphasis on the cryoprotectant properties, suitable materials and morphology. It is concluded that the formation of ice, through both direct and indirect effects, is probably fundamental to these difficulties, and this is why vitrification seems to be the most promising modality of cryopreservation.

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玻璃化是组织工程构建物低温保存的前景
冷冻保存在组织库中发挥着重要的作用,当越来越多的组织工程产品常规进入临床领域时,冷冻保存将具有更大的价值。组织工程最常见的概念是将支架(细胞固体)或基质(水凝胶)与活细胞结合,形成组织工程结构(TEC),以促进组织的修复和再生。支架和基质有望支持细胞定植、迁移、生长和分化,并指导所需组织的发育。然而,组织工程的前景取决于将产品实际分发给有需要的患者的能力。由于这个原因,低温保存细胞,tec,甚至整个实验室生产的器官的能力可能是必不可少的。低温保存可以通过传统的冷冻和玻璃化(无冰低温保存)来实现。在本出版物中,我们试图定义玻璃化tec的需求与愿望,特别强调冷冻保护剂性能,合适的材料和形态。结论是,冰的形成,通过直接和间接的影响,可能是这些困难的根本原因,这就是为什么玻璃化似乎是最有希望的冷冻保存方式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomaterials
Biomaterials 工程技术-材料科学:生物材料
CiteScore
26.00
自引率
2.90%
发文量
565
审稿时长
46 days
期刊介绍: Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice. The scope of the journal covers the wide range of physical, biological and chemical sciences that underpin the design of biomaterials and the clinical disciplines in which they are used. These sciences include polymer synthesis and characterization, drug and gene vector design, the biology of the host response, immunology and toxicology and self assembly at the nanoscale. Clinical applications include the therapies of medical technology and regenerative medicine in all clinical disciplines, and diagnostic systems that reply on innovative contrast and sensing agents. The journal is relevant to areas such as cancer diagnosis and therapy, implantable devices, drug delivery systems, gene vectors, bionanotechnology and tissue engineering.
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