{"title":"The oxidation of fatty acids combined with albumin by isolated rat-liver mitochondria in the presence of fluorocitrate","authors":"P. Björntorp","doi":"10.1016/0926-6593(66)90169-X","DOIUrl":null,"url":null,"abstract":"<div><p>Fluorocitrate was investigated as an inhibitor of the citric acid cycle which could make possible the separate study of β-oxidation of fatty acids bound to albumin using isolated rat-liver mitochondria. Fluorocitrate was shown to inhibit completely not only the CO<sub>2</sub> formation but also the incorporation of label from fatty acids into citrate in agreement with previous work. The reaction products identified were acetoacetate and β-hydroxybutyrate. No evidence for interference of fluorocitrate with β-oxidation or with phosphorylation coupled to this oxidation was found. Acetoacetate formation from fatty acids was rapid and initially linear.</p></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":"128 2","pages":"Pages 221-227"},"PeriodicalIF":0.0000,"publicationDate":"1966-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90169-X","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665936690169X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Fluorocitrate was investigated as an inhibitor of the citric acid cycle which could make possible the separate study of β-oxidation of fatty acids bound to albumin using isolated rat-liver mitochondria. Fluorocitrate was shown to inhibit completely not only the CO2 formation but also the incorporation of label from fatty acids into citrate in agreement with previous work. The reaction products identified were acetoacetate and β-hydroxybutyrate. No evidence for interference of fluorocitrate with β-oxidation or with phosphorylation coupled to this oxidation was found. Acetoacetate formation from fatty acids was rapid and initially linear.
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