The inter-relationships of low redox potential cytochrome c552 and hydrogenase in facultative anaerobes

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation Pub Date : 1966-12-14 DOI:10.1016/0926-6593(66)90002-6

J.A. Cole, J.W.T. Wimpenny

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引用次数: 41

Abstract

1.
1. Cytochrome $c_{552}$ and hydrogenase (EC 1.12.1.1) activities have been compared in Escherichia coli and Escherichia aurescens cells grown aerobically and anaerobically in both complex and defined media.
2.
2. Sodium formate added to the growth media stimulates synthesis of increased amounts of cytochrome $c_{552}$ .
3.
3. Extensive washing in 0.1 M phosphate buffer (pH 7.4), extensive sonication, and washing with 0.2% w/v sodium deoxycholate all solubilise cytochrome $c$ from hydrogenase-active membranes.
4.
4. Residues from the above procedures still show hydrogenase activity.
5.
5. Cells grown anaerobically with nitrate s terminal electron acceptor contain greatly enhanced amounts of cytochrome: these cells do not have hydrogenase, hydrogenlyase or soluble viologen-linked formic dehydrogenase activities.

The relationship of cytochrome $c_{552}$ to hydrogenase is discussed.

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兼性厌氧菌低氧化还原电位细胞色素c552与氢化酶的相互关系

1.1. 细胞色素c552和氢化酶(EC 1.12.1.1)的活性在大肠杆菌和金黄色埃希菌在复合培养基和固定培养基中厌氧和有氧培养的细胞中进行了比较。在培养基中添加甲酸钠刺激细胞色素c552.3.3的合成。在0.1 M磷酸盐缓冲液(pH 7.4)中广泛洗涤，广泛超声，并用0.2% w/v脱氧胆酸钠洗涤，均可使细胞色素c从氢化酶活性膜中溶解。以上步骤的残留物仍然显示出氢化酶活性。在硝酸末端电子受体厌氧条件下生长的细胞含有大量的细胞色素，这些细胞不具有氢化酶、氢化酶或可溶性氨基甲酸脱氢酶的活性。讨论了细胞色素c552与氢化酶的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation

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