Molecular forms of human-liver arginase

Luz Bascur, Julio Cabello, Marta Véliz, Adriana González
{"title":"Molecular forms of human-liver arginase","authors":"Luz Bascur,&nbsp;Julio Cabello,&nbsp;Marta Véliz,&nbsp;Adriana González","doi":"10.1016/0926-6593(66)90151-2","DOIUrl":null,"url":null,"abstract":"<div><p>The chromatography of liver homogenates and purified preparations of human-liver arginase (<span>l</span>-arginine ureohydrolase, EC 3.5.3.1) on CM-cellulose separates two protein fractions with arginase activity. On rechromatography, each of these fractions appears homogeneous and retains its adsorption-elution characteristics.</p><p>Both arginase fractions have similar properties as far as their affinities for substrates, pH optima and thermal inactivation are concerned. However, they differ in their pH stabilities and in their inhibition by ornithine and canavanine.</p></div>","PeriodicalId":100160,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1966-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6593(66)90151-2","citationCount":"36","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926659366901512","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 36

Abstract

The chromatography of liver homogenates and purified preparations of human-liver arginase (l-arginine ureohydrolase, EC 3.5.3.1) on CM-cellulose separates two protein fractions with arginase activity. On rechromatography, each of these fractions appears homogeneous and retains its adsorption-elution characteristics.

Both arginase fractions have similar properties as far as their affinities for substrates, pH optima and thermal inactivation are concerned. However, they differ in their pH stabilities and in their inhibition by ornithine and canavanine.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
人肝精氨酸酶的分子形式
对人肝精氨酸酶(l-精氨酸脲水解酶,EC 3.5.3.1)的肝脏匀浆和纯化制剂在cm -纤维素上进行层析,分离出具有精氨酸酶活性的两个蛋白组分。在重层析上,这些馏分中的每一个看起来都是均匀的,并保持其吸附-洗脱特性。两种精氨酸酶组分在对底物的亲和力、pH最优值和热失活方面具有相似的性质。然而,它们在pH稳定性和对鸟氨酸和大麻氨酸的抑制作用方面有所不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Author index Subject index Insect extramitochondrial glycerophosphate dehydrogenase II. Enzymic properties and amino acid composition of the enzyme from honeybee (Apis mellifera) thoraces The inter-relationships of low redox potential cytochrome c552 and hydrogenase in facultative anaerobes The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coli
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1