Morphological changes in isolated rat-liver mitochondria during swelling and contraction

Paulina Wlodawer , Donald F. Parsons , G.R. Williams , Lech Wojtczak
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引用次数: 46

Abstract

Morphological changes of rat-liver mitochondria brought about by swelling and ATP-induced contraction were studied by electron microscopy. The course of swelling is proposed to be as follows: Entering of water (and solutes) causes breakage of the outer membrane and formation of protrusion of the inner membrane. The distention of the inner membrane is accompanied by loss of density of the matrix and by disapperance and change of shape of the cristae, probably due to their unfolding and evagination. Both membranes seem to possess a limited ability to stretch.

Addition of ATP to mitochondria swollen in the presence of either orthophosphate, oleate and thyroxine gave rise to structures different from mitochondria before the onset of swelling. Restoration of the mitochondrial morphology was not observed. It is thought that the processes initiated by ATP are not a simple reversal of swelling.

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离体大鼠肝脏线粒体在肿胀和收缩过程中的形态变化
电镜观察了肿胀和atp诱导的收缩对大鼠肝脏线粒体形态的影响。膨胀的过程是这样的:水(和溶质)的进入导致外膜的破裂和内膜的突出。内膜的膨胀伴随着基质密度的丧失和嵴的消失和形状的改变,这可能是由于它们的展开和外翻。两种膜似乎都具有有限的拉伸能力。在正磷酸盐、油酸盐和甲状腺素存在的情况下,向肿胀的线粒体添加ATP会产生与肿胀发生前线粒体不同的结构。未观察到线粒体形态的恢复。据认为,ATP启动的过程不是肿胀的简单逆转。
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