Retinol and alcohol dehydrogenases in retina and liver

Ann L. Koen, Charles R. Shaw
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引用次数: 76

Abstract

Isozymes of alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.1) and retinol (vitamin A1) dehydrogenase (retinol: NAD+ oxidoreductase) were studied in retina and liver extracts of rat by starch-gel electrophoresis. The retina demonstrated two zones of retinol dehydrogenase activity and a separate zone of alcohol dehydrogenase activity. The liver had single zones each of retinol and alcohol dehydrogenase activity, neither of which coincided with the three zones from the retina. It is concluded on the basis of the electrophoretic findings supplemented with inhibition, heat inactivation, and pH studies that these five zones represent five different enzymes, and that vitamin A is oxidized in the retina and the liver by specific and distinct enzymes. Liver-type alcohol dehydrogenase is absent from the eyes and eye-type is absent from the liver.

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视网膜和肝脏中的视黄醇和醇脱氢酶
采用淀粉凝胶电泳法研究了大鼠视网膜和肝脏提取物中乙醇脱氢酶(乙醇:NAD+氧化还原酶,EC 1.1.1.1)和视黄醇(维生素A1)脱氢酶(视黄醇:NAD+氧化还原酶)的同工酶。视网膜显示两个视黄醇脱氢酶活性区和一个单独的醇脱氢酶活性区。肝脏的视黄醇和乙醇脱氢酶活性各有一个区域,两者都与视网膜的三个区域不一致。根据电泳结果、抑制、热失活和pH值研究,这五个区域代表五种不同的酶,维生素A在视网膜和肝脏中被特定和不同的酶氧化。肝型酒精脱氢酶不存在于眼睛中,眼型不存在于肝脏中。
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