In vivo studies on the regeneration kinetics of haemopoietic spleen colony-forming cells from long-term bone marrow cultures.

Cell and tissue kinetics Pub Date : 1983-07-01
J F Eliason, M Edelstein
{"title":"In vivo studies on the regeneration kinetics of haemopoietic spleen colony-forming cells from long-term bone marrow cultures.","authors":"J F Eliason,&nbsp;M Edelstein","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Production of murine haemopoietic progenitor cells, capable of forming colonies on the spleens of irradiated recipients (CFU-s), can be maintained in vitro for up to several months. We have examined the properties of the suspension (SUSP) and adherent (ADH) CFU-s from these long-term marrow cell cultures in the spleen colony assay system. The numbers of colonies formed was linear with the numbers of SUSP or ADH cells injected, although the concentrations were only 20-25% of that found in normal bone marrow (NBM). The seeding efficiencies for SUSP and ADH CFU-s, measured in spleen and femur 24 hr after transplantation, were not significantly different from those for CFU-s from NBM. The results demonstrate that the CFU-s assay can give valid estimates of colony-forming cell numbers in the cultured cell populations. We therefore used the CFU-s assay to study the regeneration of SUSP, ADH and NBM CFU-s in spleen and femur at various times after transplantation. The growth of the cultured cells appeared to be delayed by 36-48 hr compared to the regeneration of CFU-s from NBM. Thereafter, ADH CFU-s grew at the same rate as NBM CFU-s, whereas the rate of SUSP CFU-s regeneration was slightly, but not significantly, slower. This similarity in growth rates indicates that the CFU-s from long-term marrow cultures have similar self-renewal probabilities to normal CFU-s.</p>","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 4","pages":"375-83"},"PeriodicalIF":0.0000,"publicationDate":"1983-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and tissue kinetics","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Production of murine haemopoietic progenitor cells, capable of forming colonies on the spleens of irradiated recipients (CFU-s), can be maintained in vitro for up to several months. We have examined the properties of the suspension (SUSP) and adherent (ADH) CFU-s from these long-term marrow cell cultures in the spleen colony assay system. The numbers of colonies formed was linear with the numbers of SUSP or ADH cells injected, although the concentrations were only 20-25% of that found in normal bone marrow (NBM). The seeding efficiencies for SUSP and ADH CFU-s, measured in spleen and femur 24 hr after transplantation, were not significantly different from those for CFU-s from NBM. The results demonstrate that the CFU-s assay can give valid estimates of colony-forming cell numbers in the cultured cell populations. We therefore used the CFU-s assay to study the regeneration of SUSP, ADH and NBM CFU-s in spleen and femur at various times after transplantation. The growth of the cultured cells appeared to be delayed by 36-48 hr compared to the regeneration of CFU-s from NBM. Thereafter, ADH CFU-s grew at the same rate as NBM CFU-s, whereas the rate of SUSP CFU-s regeneration was slightly, but not significantly, slower. This similarity in growth rates indicates that the CFU-s from long-term marrow cultures have similar self-renewal probabilities to normal CFU-s.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
长期骨髓培养的造血脾脏集落形成细胞再生动力学的体内研究。
小鼠造血祖细胞的产生,能够在受辐照受体(CFU-s)的脾脏上形成菌落,可以在体外维持长达几个月。我们检查了悬浮(SUSP)和粘附(ADH) CFU-s从这些长期骨髓细胞培养脾集落测定系统的性质。尽管其浓度仅为正常骨髓(NBM)的20-25%,但其菌落数量与注射的SUSP或ADH细胞数量呈线性关系。移植后24小时在脾脏和股骨中测量的SUSP和ADH CFU-s的播种效率与NBM的CFU-s无显著差异。结果表明,CFU-s试验可以有效地估计培养细胞群中集落形成细胞的数量。因此,我们采用CFU-s法研究移植后不同时间脾脏和股骨中SUSP、ADH和NBM CFU-s的再生情况。与NBM的CFU-s再生相比,培养细胞的生长似乎延迟了36-48小时。此后,ADH CFU-s的生长速度与NBM CFU-s相同,而SUSP CFU-s的再生速度略慢,但不明显。这种生长速度的相似性表明,长期骨髓培养的CFU-s与正常CFU-s具有相似的自我更新概率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Abstracts of the joint meeting of the Cell Kinetics Society and the International Cell Cycle Society. 28-31 March 1990, St Louis, Missouri, U.S.A. Abstracts of the 16th meeting of the European Study Group for Cell Proliferation. 3-6 May 1989, Milan. Proceedings of the Cell Kinetics Society, thirteenth annual meeting. 29 March-1 April 1989, White Plains, New York, U.S.A. Bone marrow fibroblast colony-forming cells are osteogenic stem cells. Epidermal tissue homeostasis. III. Effect of hydrocortisone on cell pool size, cell birth rate and cell loss in normal toads and in toads deprived of the pars distalis of the pituitary gland.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1