{"title":"Effect of ions on alpha-amylase activity of human granulocytes.","authors":"I Zakrzewska","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of the salts NaCl, KCl, CsCl, NH4Cl, NaBr, CH3COONa, NaNO3 and Na2CO3 on the alpha-amylase activity of dialysed and nondialysed human granulocyte extracts was measured. The optimum activity was found at the 0.02 M salt concentrations. The highest enzyme activity was found with NaCl, CsCl, NH4Cl and KCl. The apoamylase was successfully reactivated with the cations Ca2+, Ba2+, Mn2+, while reactivations with Co2+, Ni2+, Mg2+ are below the limits of significance.</p>","PeriodicalId":7056,"journal":{"name":"Acta biologica Academiae Scientiarum Hungaricae","volume":"33 1","pages":"55-9"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta biologica Academiae Scientiarum Hungaricae","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The effect of the salts NaCl, KCl, CsCl, NH4Cl, NaBr, CH3COONa, NaNO3 and Na2CO3 on the alpha-amylase activity of dialysed and nondialysed human granulocyte extracts was measured. The optimum activity was found at the 0.02 M salt concentrations. The highest enzyme activity was found with NaCl, CsCl, NH4Cl and KCl. The apoamylase was successfully reactivated with the cations Ca2+, Ba2+, Mn2+, while reactivations with Co2+, Ni2+, Mg2+ are below the limits of significance.
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