Type I DNA topoisomerases from mammalian cell nuclei interlock strands and promote renaturation of denatured closed circular PM2 DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-09-28 DOI:10.1016/0005-2787(81)90010-1

Paul P. Lau , Horace B. Gray Jr. , Chik-Fong Wei , Randy J. Legerski , Donald L. Robberson

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引用次数: 2

Abstract

Type I DNA topoisomerases from mouse ascites cell nuclei and from rat liver cell nuclei act on denatured viral closed circular PM2 DNA to produce molecules with a highly contracted structure as well as fully duplex nonsupercoiled covalently closed circular molecules. Highly contracted DNA molecules contain a novel type of topological linkage in which a strand in one region of the double-stranded molecule passes between the strands in another region of the circular molecule one or more times. Since it is also found that the action of the topoisomerase promotes renaturation of complementary strands in denatured closed circular DNA, it is suggested that formation of contracted DNA structures proceeds through renatured, duplex intermediates with highly negative superhelix densities that contain small single-stranded regions.

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来自哺乳动物细胞核的I型DNA拓扑异构酶互锁链并促进变性闭合环状PM2 DNA的再生

来自小鼠腹水细胞核和大鼠肝细胞核的I型DNA拓扑异构酶作用于变性病毒闭合环状PM2 DNA，产生具有高度收缩结构的分子以及全双工非超卷共价闭合环状分子。高度收缩的DNA分子包含一种新型的拓扑链接，其中双链分子的一个区域的链在圆形分子的另一个区域的链之间传递一次或多次。由于还发现拓扑异构酶的作用促进了变性闭合环状DNA中互补链的再生，因此表明收缩DNA结构的形成是通过具有高度负超螺旋密度的再生双相中间体进行的，这些中间体含有小的单链区域。

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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis

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