Preparation and specificity of antibodies directed toward the ribose methylated nucleotide, 2′-O-methylguanosine 5′-monophosphate

Abstract

Antibodies have been produced that recognize the ribose methylated nucleotide, $\text{2′-O-}\text{methylguanosine}$ 5′-monophosphate, pGm. Specificity was tested in a radioimmunoassay. Other ribose methylated nucleotides, deoxyguanosine, and guanosine 5′-monophosphate exhibited negligible cross-reactivity. Elements of antibody recognition, in descending order of importance, were the parent base, the methylated ribose moiety, and the phosphate group.