Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment

Ben A. Oostra, Annika C. Arnberg, Geert Ab, Max Gruber
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引用次数: 6

Abstract

When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of Escherichia coli, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.

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转录截断DNA片段的大肠杆菌RNA聚合酶的末端链转换
当转录含有大肠杆菌启动子和rrnE操纵子第一部分的限制性内切片段时,RNA聚合酶全酶只在启动子上开始。除了径流转录本外,末端链开关还可形成比模板大的分子。
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