Amino acid sequence determination of the ADP,ATP carrier from beef heart mitochondria

Abstract

The primary structure of the C-terminal region (94 residues) of the ADP,ATP carrier of beef heart mitochondria is described. CNBr cleavage results in a large peptide (CB1) with $\text{M}{}_{\mathrm{<mtext>r</mtext>}}$ 22 000 and several small peptides (CB2 to CB8). Peptide separation was achieved by gel chromatography with 80% formic acid or with an ethanol/formic acid mixture. The amino acid sequence of the small CNBr peptides was determined by solid-phase techniques. Hydrolysis in formic acid cleaves the carrier protein into an $\text{M}{}_{\mathrm{<mtext>r</mtext>}}$ 23 000 fragment (A1) with the blocked N-terminus and an $\text{M}{}_{\mathrm{<mtext>r</mtext>}}$ 10 000 fragment (A2) starting with proline. The alignment of two CNBr fragments was possible by degradation of A2 by solid-phase methods for 34 steps. The remaining CNBr fragments were arranged by sequencing the tryptic peptides of citraconylated A2.