Studies on the primary structures of the exocellular d-alanyl-d-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39

Colette Duez , Jean-Marie Frère , Jean-Marie Ghuysen , Jozef Van Beeumen , Joël Vandekerckhove
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引用次数: 5

Abstract

The Mr 37 000 d-alanyl-d-alanine peptidase excreted by Streptomyces R61 and the Mr 530 00 d-alanyl-d-alanine peptidase excreted by Actinomadura R39 are both characterized by a very uneven distribution of the basic (Arg + Lys) amino acid residues. Trypsin degradation of the heat-denatured enzymes generates (1) thirteen soluble peptides which contain from 2 to 28 residues in the case of the R61 enzyme and nineteen soluble peptides which contain 2 to 39 residues in the case of the R39 enzyme; and (2) three large segments or core peptides which, irrespective of the enzymes from which they originate, consist of 50–60, 70–80 and 110–120 residues. About 90% of the basic (Arg + Lys) amino acid residues are recovered in the soluble tryptic peptides. The core peptides represent 62% (Mr ≈ 23 000) and 45% (Mr ≈ 24 000) of the untreated R61 and R39 enzymes, respectively. One 28-residue soluble peptide isolated from the R61 enzyme represents the N-terminal portion of the protein whose sequence has been established. The penicillin attachment site of the R61 enzyme has been located in one of the core peptides. For the R39 enzyme, indirect evidence shows that the penicillin binding site is probably within one of the soluble peptides.

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链霉菌R61和放线瘤菌R39胞外d-丙烯酰-d-丙氨酸肽酶初级结构的研究
链霉菌R61分泌的d-alanyl-d-丙氨酸肽酶分子量为37000,放线瘤菌R39分泌的d-alanyl-d-丙氨酸肽酶分子量为530 000,它们的碱性(Arg + Lys)氨基酸残基分布非常不均匀。胰蛋白酶降解热变性酶产生(1)13个可溶性肽,在R61酶的情况下含有2至28个残基,在R39酶的情况下含有19个可溶性肽,含有2至39个残基;(2)三个大片段或核心肽,无论它们源自哪种酶,由50-60、70-80和110-120个残基组成。大约90%的碱性(精氨酸+赖氨酸)氨基酸残基在可溶性色氨酸肽中被回收。核心肽分别代表62% (Mr≈23000)和45% (Mr≈24000)的未处理R61和R39酶。从R61酶中分离出一个28个残基的可溶性肽,代表该蛋白的n端部分,其序列已经确定。R61酶的青霉素附着位点位于其中一个核心肽中。对于R39酶,间接证据表明青霉素结合位点可能在其中一个可溶性肽内。
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Author index Errata Protamine interacts with the D-domains of fibrinogen Studies on the primary structures of the exocellular d-alanyl-d-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39 Resonance Raman spectra of deoxyhemoproteins. Heme structure in relation to dioxygen binding
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