[Interdependence of mycelial dehydrogenase activity in Streptomyces aureofaciens and its capacity for tetracycline biosynthesis].

Antibiotiki Pub Date : 1984-10-01
L M Novikova, V G Makarevich
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Abstract

The maximum dehydrogenase (oxidation-reduction) activity of the vegetative inoculum of S. aureofaciens is the criterion of its availability for transfer to the fermentation medium. The activity is determined by the rate of the methylen blue reduction and the growth characteristics of the seed culture. A method for estimation of the mycelium amount in the medium containing insoluble solid particles is described. The method is based on the capacity of the tetracycline-producing organism for decoloration of methylen blue.

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[金黄色链霉菌菌丝脱氢酶活性与其四环素生物合成能力的相互依赖性]。
金黄色葡萄球菌营养接种物的最大脱氢酶(氧化还原)活性是其能否转移到发酵培养基的标准。活性是由亚甲基蓝还原速率和种子培养的生长特性决定的。描述了一种在含有不溶性固体颗粒的培养基中估计菌丝体数量的方法。该方法基于产生四环素的生物对亚甲基蓝的脱色能力。
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