[Nutrient medium for determining the biological activity of heliomycin].

Antibiotiki Pub Date : 1984-10-01
E M Bershteĭn, N G Vasil'eva, M S Poliak, V M Grigor'eva, L N Astanina
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Abstract

It was shown that a nutrient medium containing only salt components except agar-agar could be used for assay of the biological activity of heliomycin. The optimal results were observed on the medium containing ammonium chloride, urea, disubstituted sodium phosphate, glucose and agar-agar. The medium provided satisfactory growth of Bac. subtilis ATCC 6633 as the test culture and formation of clear inhibition growth zones of the sufficient size. The medium is standard and its use allows obtaining reproducible results in assay of the heliomycin biological activity.

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[测定heliomycin生物活性的营养培养基]。
结果表明,除琼脂外,仅含盐组分的营养培养基可用于测定heliomycin的生物活性。在含氯化铵、尿素、二取代磷酸钠、葡萄糖和琼脂的培养基上观察到最佳效果。培养基提供了令人满意的Bac生长。以枯草杆菌ATCC 6633为试培养物,形成了足够大小的明显抑制生长带。该培养基是标准的,它的使用允许在测定heliomycin生物活性中获得可重复的结果。
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