Histochemical study of mucopolysaccharides in epidermal mucous cells and subjacent granular cells of Glossoscolex uruguayensis L. (Righi, 1978).

Folia histochemica et cytochemica Pub Date : 1981-01-01
V D Dall Pai, I R Santos Costa, A C Pacheco, C E Alves, N Macha
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Abstract

Histochemical study of mucopolysaccharides of both mucous secreting and subjacent granular cells of the epidermis of Glossoscolex uruguayensis L (Righi, 1978), has been done. Histochemical tests revealed two major types of mucous cells: large ortochromatic mucous cells and metachromatic mucous cells. The first ones possess alpha-amylase resistant, PAS-positive material, together with little mucic acid, and the latter present a very low rate of neutral mucopolysaccharides and to a variable degree, both carboxylate and sulphate mucopolysaccharides. A third cell type was also detected, presenting a frothy acid like material. Subjacent epidermal granular cells display alpha-amylase resistant, PAS-positive granules, with no acid mucus.

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乌拉圭舌骨虫表皮黏液细胞和下颗粒细胞中粘多糖的组织化学研究(Righi, 1978)。
对乌拉圭舌骨虫(Glossoscolex uruguayensis L)表皮的粘液分泌细胞和下皮层颗粒细胞的粘多糖进行了组织化学研究(Righi, 1978)。组织化学检查显示两种主要类型的黏液细胞:大的异色黏液细胞和异色黏液细胞。前者具有α -淀粉酶抗性,pas阳性物质,并且含有少量的黏液酸;后者含有极低比例的中性黏液多糖,并且在不同程度上含有羧酸和硫酸盐黏液多糖。第三种细胞类型也被检测到,呈现出一种泡沫状的酸状物质。下表皮颗粒细胞显示α -淀粉酶抗性,pas阳性颗粒,无酸性粘液。
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