Visceral larva migrans: examinations by means of enzyme-linked immunosorbent assay of human sera for antibodies to excretory-secretory antigens of the second-stage larvae of Toxocara canis.

Zeitschrift fur Parasitenkunde (Berlin, Germany) Pub Date : 1983-01-01 DOI:10.1007/BF00934015

F van Knapen, J van Leusden, A M Polderman, J H Franchimont

{"title":"Visceral larva migrans: examinations by means of enzyme-linked immunosorbent assay of human sera for antibodies to excretory-secretory antigens of the second-stage larvae of Toxocara canis.","authors":"F van Knapen, J van Leusden, A M Polderman, J H Franchimont","doi":"10.1007/BF00934015","DOIUrl":null,"url":null,"abstract":"<p><p>An enzyme-linked immunosorbent assay is described for the detection of serum antibodies to visceral larva migrans (Toxocariasis). Excretory-secretory antigens of the second-stage larvae of Toxocara canis were used as antigen to coat the polystyrene plates. With sera from patients high antibody titers were observed in both ocular and visceral disorders. Cross-reactions due to other parasitic infections could be excluded, including other migrating larval infections such as ascariasis, trichinellosis, strongyloidiasis, filariasis, and anisakiasis. In a small seroepidemiologic survey of healthy primary schoolchildren, a remarkably high percentage (7.1) reacted positively to this method. These children showed eosinophilia as compared to the seronegative group. The data were compared with those observed in other countries and the results prompt reconsideration of the significance of T. canis for public health.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"69 1","pages":"113-8"},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00934015","citationCount":"30","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF00934015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 30

Abstract

An enzyme-linked immunosorbent assay is described for the detection of serum antibodies to visceral larva migrans (Toxocariasis). Excretory-secretory antigens of the second-stage larvae of Toxocara canis were used as antigen to coat the polystyrene plates. With sera from patients high antibody titers were observed in both ocular and visceral disorders. Cross-reactions due to other parasitic infections could be excluded, including other migrating larval infections such as ascariasis, trichinellosis, strongyloidiasis, filariasis, and anisakiasis. In a small seroepidemiologic survey of healthy primary schoolchildren, a remarkably high percentage (7.1) reacted positively to this method. These children showed eosinophilia as compared to the seronegative group. The data were compared with those observed in other countries and the results prompt reconsideration of the significance of T. canis for public health.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

内脏幼虫迁移:用酶联免疫吸附法检测人血清对犬弓形虫第二阶段幼虫排泄-分泌抗原的抗体。

一种酶联免疫吸附测定法用于检测内脏幼虫迁移(弓形虫病)的血清抗体。以犬弓形虫二期幼虫的排泄-分泌抗原为抗原包被聚苯乙烯板。在眼病和内脏疾病患者血清中均观察到高抗体滴度。可排除其他寄生虫感染引起的交叉反应，包括其他迁徙幼虫感染，如蛔虫病、旋毛虫病、圆线虫病、丝虫病和异丝虫病。在对健康小学生进行的一项小型血清流行病学调查中，对这种方法有积极反应的比例非常高(7.1)。与血清阴性组相比，这些儿童表现出嗜酸性粒细胞增多。这些数据与在其他国家观察到的数据进行了比较，结果促使人们重新考虑犬弓形虫对公共卫生的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Zeitschrift fur Parasitenkunde (Berlin, Germany)

自引率

0.00%

发文量