Use of trinitrobenzenesulfonic acid in the determination of protein amino groups on the external surface of rat erythrocyte membranes.

B S Fomenko, I E Dovgii, I G Akoev
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Abstract

Description is provided of conditions selected for the study of protein amino groups (AG) on the external surface of rat erythrocyte membranes (EM) by means of 2,4,6-trinitrobenzenesulfonic acid (TNBS). The number of protein AG reacting with TNBS was influenced by the season of the year, the physiologic state of the donor animal, and other factors known to alter EM. It appears that structural perturbations introduced by these factors into EM are responsible for alterations in the accessibility of the protein AG to TNBS.

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用三硝基苯磺酸法测定大鼠红细胞膜外表面的蛋白质氨基。
描述了用2,4,6-三硝基苯磺酸(TNBS)研究大鼠红细胞膜外表面蛋白质氨基(AG)的条件选择。与TNBS发生反应的AG蛋白的数量受到季节、供体动物的生理状态和其他已知改变EM的因素的影响。这些因素引入EM的结构扰动似乎是导致AG蛋白对TNBS可及性改变的原因。
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