{"title":"Isolation of glycogen from buffalo liver.","authors":"P K Agrawal, U K Misra","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The glycogen has been isolated from buffalo (Bos Bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. Once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. The buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with DEAE-cellulose.</p>","PeriodicalId":8818,"journal":{"name":"Biochemistry and experimental biology","volume":"16 3","pages":"251-5"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and experimental biology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The glycogen has been isolated from buffalo (Bos Bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. Once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. The buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with DEAE-cellulose.