Isolation of glycogen from buffalo liver.

P K Agrawal, U K Misra
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Abstract

The glycogen has been isolated from buffalo (Bos Bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. Once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. The buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with DEAE-cellulose.

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水牛肝中糖原的分离。
从水牛(Bos Bubalus bubalis)肝脏中分离得到糖原,经多次纯化,并与兔糖原和牡蛎糖原进行了比较。曾经纯化过的水牛糖原,蛋白质含量为1.3%,氮含量为0.16%,磷含量为0.69%,不含脂质,核酸含量足以在260亩的土壤中吸收。经过两次或三次纯化和deae -纤维素处理后,水牛糖原可以作为兔糖原和牡蛎糖原的潜在替代品。
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