Organization of highly purified calf thymus DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-10-27 DOI:10.1016/0005-2787(81)90039-3

Richard S. Welsh, Karel Vyska

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引用次数: 16

Abstract

DNA (N-DNA) prepared under conditions eliminating the exposure of chromatin to cytoplasmic components exhibits some special properties not observed for DNA prepared by standard methods (S-DNA). N-DNA, having a sedimentation coefficient of 24.7 S and a firmly bound protein content of 0.7%, can be cleaved (in contrast to S-DNA) by treatment with chelating agents, into stable subunits having a mean molecular weight of about 500 000. This cleavage was shown to be an ordered process which involved no enzymatic or shear degradation. It was accompanied by the release of phosphopeptides. The analyses of these phosphopeptides revealed the presence of two main fractions. One contained phosphoserine and glycine ( $M_{r}$ about 1 400), and the other contained phosphoserine, glycine, alanine, glutamic and aspartic acids ( $M_{r}$ about 900). The amount of released phosphopeptides could be correlated to the extent of cleavage.

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组织高度纯化的小牛胸腺DNA

在消除染色质暴露于细胞质组分的条件下制备的DNA (N-DNA)表现出一些用标准方法制备的DNA (S-DNA)所没有的特殊性质。N-DNA的沉降系数为24.7 S，结合蛋白含量为0.7%，与S- dna相比，通过螯合剂的处理，N-DNA可以被切割成平均分子量约为50万的稳定亚基。这种解理被证明是一个有序的过程，不涉及酶或剪切降解。它伴随着磷酸肽的释放。对这些磷酸肽的分析揭示了两个主要部分的存在。一种含有磷丝氨酸和甘氨酸(Mr约为1 400)，另一种含有磷丝氨酸、甘氨酸、丙氨酸、谷氨酸和天冬氨酸(Mr约为900)。磷脂肽的释放量可能与裂解的程度有关。

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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis

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