{"title":"A tentative elucidation of the sequence of human triosephosphate isomerase by homology peptide mapping","authors":"Pau M. Yuan, John M. Talent, Robert W. Gracy","doi":"10.1016/0005-2795(81)90136-7","DOIUrl":null,"url":null,"abstract":"<div><p>By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.</p></div>","PeriodicalId":100165,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Protein Structure","volume":"671 2","pages":"Pages 211-218"},"PeriodicalIF":0.0000,"publicationDate":"1981-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2795(81)90136-7","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Protein Structure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005279581901367","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
Abstract
By utilizing homologous peptide mapping and amino acid analysis of recovered peptides, it has been possible to infer much of the sequence of human triosephosphate isomerase. This has been achieved with less than 5 mg of the human enzyme. The enzyme shows a great sequence homology with the rabbit enzyme. The methods used in this study can provide a rapid procedure for gaining structural information on human enzymes available only in microgram or milligram quantities.
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