Purification of lymphocystis disease virus (LDV) grown in tissue culture. Evidences for the presence of two types of viral particles.

Abstract

Lymphocystis disease virus was highly purified from host cells by precipitation with PEG-6000 and isopycnic centrifugation in a metrizamide gradient. Metrizamide gradient centrifugation produce two distinct bands at equilibrium. As calculated from reconstruction experiments, only 4 and 0.3% respectively of the host DNA and the host proteins were recovered at the position of the bands. The final recovery of infectivity was about 41%. Electron microscopy of the bands showed two types of particles: small and dense particles measuring 100-150 nm and lymphocystis virions that measured about 300-350 nm in diameter.