Proliferative effect of burst-promoting activity upon early erythroid progenitors.

K G Brockbank
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Abstract

The mechanism of action of burst-promoting activity (BPA) was investigated. Rabbit bone marrow conditioned medium (BMCM) was used as a source of BPA for bone marrow derived rabbit erythroid progenitors (BFU-e). The addition of 10% BMCM to methylcellulose cultures resulted in an increase in both burst number (60%) and 59Fe incorporation into heme (1,334%). A strong correlation was observed between cell number and 59 Fe incorporation individual bursts. Analysis of cell number in individual bursts cultured in the presence or absence of BMCM demonstrated that BMCM consistently enhanced burst size. When bone marrow cells were incubated for 4 hours in BMCM prior to culture, a three fold increases in the percentage of S-phase BFU-e was observed. These results show that a major mechanism of action of BMCM burst-promoting activity is to enhance proliferation during the early phase of burst formation.

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促爆活性对早期红系祖细胞的增殖作用。
探讨了促爆活性(BPA)的作用机制。采用兔骨髓条件培养基(BMCM)作为骨髓源兔红细胞祖细胞(BFU-e)双酚a的来源。在甲基纤维素培养物中添加10%的BMCM,使爆发数增加了60%,血红素中的59Fe掺入量增加了1334%。观察到细胞数量与59铁掺入个体爆发之间有很强的相关性。对存在或不存在BMCM培养的单个爆发细胞数量的分析表明,BMCM始终增强爆发大小。骨髓细胞在培养前在BMCM中孵育4小时,观察到s期BFU-e的百分比增加了3倍。这些结果表明,BMCM促进爆发活性的主要作用机制是在爆发形成的早期促进增殖。
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