Cognate interaction between human lymphocytes and eosinophils is mediated by beta 2-integrins and very late antigen-4.

H J Mengelers, T Maikoe, J A Raaijmakers, J W Lammers, L Koenderman
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Abstract

Here the cognate interactions between human eosinophils and lymphocytes are studied. These interactions were measured in a double-colored FACS analysis by applying fluorescent red eosinophils (stained with hydroethidine, 40 mumol/L) and fluorescent green lymphocytes (stained with sulfidofluorescein diacetate, 100 mumol/L) in a ratio of 1:3. When normal eosinophils were mixed with a total lymphocyte preparation in stirred suspensions (37 degrees C), no physical interaction was present between both cell types. However, the addition of phytohemagglutinin and PMA resulted in a clear aggregation response between both cell types (up to 30% of the eosinophils interacted with lymphocytes after 15 minutes). CD8(+)- and CD4(+)-positive T cells contributed equally to the heterotypic aggregation response. It is interesting that when lymphocytes were pretreated with phytohemagglutinin or eosinophils with phorbol myristate acetate and subsequently washed, the cells still interacted with unstimulated counterparts. The heterotypic interaction between lymphocytes and eosinophils is blocked by monoclonal antibodies directed against the beta-chain of the beta 2-integrins (CLB LFA1/1; CD18) and the alpha-chain of very late antigen-4 (VLA-4) (HP2/1; CD49d), indicating that both the beta 2-integrins and VLA-4 contributed to this heterotypic interaction. When eosinophils bound to PHA-treated lymphocytes, the cells exhibited an activated phenotype that was characterized by an enhanced expression of CD66b and CD11b. The cells interacted with each other provided that an intact cellular metabolism was present--that is, no interaction was seen after treatment with the glycolysis inhibitor sodium mono-iodoacetate.(ABSTRACT TRUNCATED AT 250 WORDS)

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人类淋巴细胞和嗜酸性粒细胞之间的同源相互作用是由β 2整合素和非常晚抗原-4介导的。
这里研究了人类嗜酸性粒细胞和淋巴细胞之间的同源相互作用。在双色FACS分析中,以1:3的比例应用荧光红色嗜酸性粒细胞(用氢乙二胺染色,40 μ mol/L)和荧光绿色淋巴细胞(用双醋酸硫荧光素染色,100 μ mol/L)来测量这些相互作用。当将正常嗜酸性粒细胞与总淋巴细胞制剂混合在搅拌悬浮液中(37℃)时,两种细胞类型之间没有物理相互作用。然而,植物血凝素和PMA的加入导致两种细胞类型之间明显的聚集反应(15分钟后高达30%的嗜酸性粒细胞与淋巴细胞相互作用)。CD8(+)-和CD4(+)阳性T细胞对异型聚集反应的贡献相同。有趣的是,当淋巴细胞用植物血凝素或嗜酸性粒细胞与肉豆酸酯phorbol醋酸预处理并随后洗涤时,细胞仍然与未刺激的细胞相互作用。淋巴细胞和嗜酸性粒细胞之间的异型相互作用被针对2-整合素(CLB LFA1/1;CD18)和极晚期抗原-4 (VLA-4) α链(HP2/1;CD49d),表明β 2整合素和VLA-4都参与了这种异型相互作用。当嗜酸性粒细胞与pha处理的淋巴细胞结合时,细胞表现出活化的表型,其特征是CD66b和CD11b的表达增强。细胞之间相互作用,前提是细胞代谢完整,也就是说,在糖酵解抑制剂单碘乙酸钠治疗后,没有发现相互作用。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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