{"title":"Ex vivo expansion of hematopoietic progenitor cells in human cord blood: an effect enhanced by cord blood serum.","authors":"H Kurata, K Takakuwa, K Tanaka","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The concept of ex vivo expansion of the human stem/progenitor cell population was tested in this study. Cord blood mononuclear cells were isolated and cultured in the presence of SCF, IL-3, and either cord blood serum (CBS) or fetal calf serum (FCS). After 8 days of incubation with CBS or FCS, the number of CFU-GM was increased ninefold and four-and-a-half-fold, respectively. However, the enhancing effect lasted for only a short period. This study demonstrates that CBS could contain growth factors expanding progenitor cells. Whether the ability of CBS to enhance the CFU-GM expanding capacity is attributable to a novel factor or factors, or represents effects of other known cytokines, remains to be determined.</p>","PeriodicalId":77160,"journal":{"name":"Hematologic pathology","volume":"9 2","pages":"73-8"},"PeriodicalIF":0.0000,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hematologic pathology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The concept of ex vivo expansion of the human stem/progenitor cell population was tested in this study. Cord blood mononuclear cells were isolated and cultured in the presence of SCF, IL-3, and either cord blood serum (CBS) or fetal calf serum (FCS). After 8 days of incubation with CBS or FCS, the number of CFU-GM was increased ninefold and four-and-a-half-fold, respectively. However, the enhancing effect lasted for only a short period. This study demonstrates that CBS could contain growth factors expanding progenitor cells. Whether the ability of CBS to enhance the CFU-GM expanding capacity is attributable to a novel factor or factors, or represents effects of other known cytokines, remains to be determined.
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