Solubilization of neuropathy target esterase and other phenyl valerate carboxylesterases from chicken embryonic brain by phospholipase A2.

J Seifert, B W Wilson
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Abstract

Membrane-bound neuropathy target esterase (NTE) and associated phenyl valerate carboxylesterases were solubilized from chicken embryo brain by phospholipase A2. Phospholipase A2 from bee or cobra (Naja) venoms were the most effective preparations in solubilizing brain NTE and other phenyl valerate carboxylesterases. Phospholipase C and several proteinases (endoproteinase, pronase E, proteinase K, thermolysin, trypsin) did not solubilize brain membrane-bound carboxylesterases but reduced their activity. NTE solubilization by phospholipase A2 did not affect its apparent Km and Vmax for the substrate phenyl valerate or the susceptibility of phenyl valerate carboxylesterases to inhibition by paraoxon and mipafox. NTE thermal stability diminished after the treatment of brain membrane fragments with phospholipase A2.

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磷脂酶A2对鸡胚脑神经病变靶酯酶及其他戊酸苯羧酸酯酶的溶解作用。
用磷脂酶A2从鸡胚脑中溶解膜结合神经病靶酯酶(NTE)和相关的戊酸苯羧酸酯酶。从蜜蜂和眼镜蛇(Naja)毒液中提取的磷脂酶A2是溶解脑NTE和其他戊酸苯羧酸酯酶最有效的制剂。磷脂酶C和几种蛋白酶(内源性蛋白酶、蛋白酶E、蛋白酶K、热溶酶、胰蛋白酶)不能溶解脑膜结合羧酸酯酶,但能降低其活性。磷脂酶A2对NTE的增溶作用不影响底物戊酸苯酯的表观Km和Vmax,也不影响戊酸苯酯羧酸酯酶对对氧磷和米帕福的抑制敏感性。磷脂酶A2处理脑膜碎片后,NTE热稳定性下降。
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